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为便于临床应用肺癌ｐ５３蛋白表达免疫组化检测方法，对３３例手术切除肺癌新鲜组织，分别制备组织印片及冰冻切片标本，用免疫组化ＡＢＣ法检测ｐ５３蛋白表达。结果：组织印片和冰冻切片的ｐ５３蛋白表达阳性率分别为６０．６０％和６３．６４％，２种方法的相关非常显著（Ｐ＜０．００１），检测结果差异不显著（Ｐ＞０．０５）。结果提示组织印片法检测ｐ５３蛋白有无异常表达简便、准确、可靠，不需做病理切片，有一定的临床应用前景。
In order to facilitate the clinical application of immunohistochemical detection of p53 protein expression in lung cancer, 33 cases of fresh lung tissue were surgically resected. Tissue prints and frozen section specimens were prepared. The expression of p53 protein was detected by immunohistochemical ABC method. Results: The positive rates of p53 protein expression in tissue print and frozen section were 60.60% and 63.64%, respectively, and the correlation between the two methods was very significant (P<0.001). The difference was not significant (P>0). .05). The results suggest that the tissue printing method is convenient, accurate and reliable for detecting abnormal expression of p53 protein, and no need to do pathological section, which has certain clinical application prospects.