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目的对成年大鼠窦房结(SAN)进行定位和形态学观察,为心脏传导系统的研究提供依据。方法采用二级Wistar成年大鼠20只,对右心房及与之相连的近段上腔静脉做水平连续切片,对切片行HE、Masson染色,光镜下观察连续切片,定位SAN并观察组织结构。结果成年大鼠SAN位于上腔静脉与右心房交界区及交界区以上的上腔静脉近段壁内,呈马蹄形/C形。大鼠SAN组织结构疏松,间质胶原纤维含量丰富,主要含有P细胞、T细胞和少量心房肌细胞。结论成年大鼠SAN位置、形态提示我们取材时一定要保留近段上腔静脉。
Objective To investigate the localization and morphology of the sinoatrial node (SAN) in adult rats and provide basis for the study of cardiac conduction system. Methods Twenty adult Wistar rats were used in this study. The right atrium and its proximal superior vena cava were serially sectioned. The sections were stained with HE and Masson, and the sections were observed under light microscope. The SAN was located and the tissue structure was observed . Results The adult rat SAN was located in the proximal segment of the superior vena cava located at the junction of the superior vena cava and the right atrium and above the junctional zone, showing a horseshoe / C shape. SAN tissue loose structure, interstitial collagen content is rich, mainly contains P cells, T cells and a small amount of atrial myocytes. Conclusion The location and morphology of SAN in adult rats suggest that the superior vena cava must be preserved in our study.