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[目的]检测骨髓间充质干细胞(BMSCs)源性内皮细胞功能基因表达水平,为骨组织工程血管化奠定基础.[方法]采用全骨髓贴壁法对BMSCs进行体外培养、纯化和扩增,用含有VEGF(10 ng·ml~(-1))和bFGF(2 ng·ml~(-1))的诱导培养液对其进行体外诱导分化3周,通过透射电镜观察内皮细胞特异性W-P小体鉴定细胞性质.以主动脉内皮细胞(VECs)为阳性对照,采用实时定量PCR(RT-qPCR)检测BMSCa源性内皮细胞一氧化氮合酶(eNOS)与酪氨酸激酶受体(Tie-2)mRNA的相对表达量.[结果]分化后的细胞在光镜下具有内皮细胞的形态学特征;透射电镜观察显示诱导后的细胞内出现内皮细胞特异性W-P小体.RT-qPCR检测显示:实验组Tie-2mR-NA的相对表达量为0.785,eNOS mRNA的相对表达量为0.747,二者与阳性对照组目的基因的表达量没有明显差异(P>0.05).[结论]BMSCs能够成功体外分化为内皮细胞,且具有成熟内皮细胞功能基因的表达,是构建血管化组织工程骨的理想种子细胞.“,”[Objective]To detect the functional gene expression of endothelial cells derived from the bone marrow mesen chymal stem cells ( BMSCs) , so as to provide the promising strategies for the establishment of vascularized tissue - engineered bone. [Methods]BMSCs were cultured, purified, and expanded by bone marrow cell adherence in vitro. They were induced and differentiated in the medium of 10ng ·ml~(-1) VEGF and 2 ng ·ml~(-1) bFGF for 3 weeks. The differentiated cells were identified for Weible - palade corpuscle in the cytoplasm by transmission electron microscopy. The relative gene expression of eNOS and Tie - 2in the BMSCs - derived endothelial cells were detected by real - time quantitative reverse transcriptase PCR ( RT -qPCR) , with the vascular endothelial cells as the positive controls. [Results]The differentiated cells demonstrated the characters of endothelial cells under phase contrast microscope. W - P corpuscles could be seen under transmission electron microscope. RT - qPCR analysis showed that the differentiated cells expressed Tie - 2 mRNA (0. 785) and eNOS mRNA (0. 747) in the trial group, but no difference was seen in BMSCs - derived endothelial or vascular endothelial cells (P > 0. 05) . [Conclusion]BMSCs could be successfully differentiated into endothelial cells in vitro, and the functional genes were stably expressed in BMSCs - derived endothelial cells. They are ideal donor cells of tissue engineering vascularization.