蚕沙提取叶绿素之研究 Ⅰ蚕沙提取叶绿素及叶绿素诱导体之鑑定

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一、蚕沙(包括蚕粪及残桑)是制取叶绿素及叶绿素铜钠盐的新原料,通过综合利用还可以制成其他产品,较用作饲料及肥料的经济价值提高30倍。二、蚕沙的色素与桑叶相似,经纸上层析法分离得6种色素,它们的顺序由下而上是:叶绿素b、叶绿素a,叶黄素。三色堇黄素、叶黄氧茂素及β葫蘿蔔素,是测定它们的Rf值。三、用光电比色法及分光光度法测定蚕沙及桑叶的叶绿素含量;蚕沙(干物)合叶绿素1.1205—1.2640%,桑叶合叶绿素1.0640—1.1375%,以春季的桑叶及蚕沙的叶绿素较多。四、蚕沙叶绿素制成水溶性叶绿素铜钠盐质量合符规格,纯度88—82%,相对消光度为3.4—4,得率0.8—2.05%。五、蚕沙叶绿素的吸收光譜与桑叶相似,而叶绿素纳盐的吸收光带偏于两极,卽6800—6500A°及4760—4000A°之间,蚕沙叶绿素的波长—消光曲线有两个吸收极大,叶绿素铜纳盐的波长—消光曲线的吸收极大为4070A°及6330A° First, silkworm sand (including silkworm excrement and residual mulberry) is a new raw material for preparation of chlorophyll and sodium copper chlorophyll. Through comprehensive utilization, it can also be made into other products. The economic value of using silkworm feces and fertilizer is 30 times higher than that of feed and fertilizer. Second, the pigment of the silkworm sand is similar to the mulberry leaf, and six kinds of pigments are separated by chromatography on the paper. Their order is from bottom to top: chlorophyll b, chlorophyll a, and lutein. Pansyrin, lutein, and beta carotene were used to determine their Rf values. Thirdly, the chlorophyll content of silkworm sand and mulberry leaves was determined by photoelectric colorimetry and spectrophotometry; silkworm sand (dry matter) combined chlorophyll 1.1205-1.2640%, mulberry leaf chlorophyll 1.0640-1.1375%, and spring mulberry leaves and silkworm sand More chlorophyll. Fourth, silkworm chlorophyll made of water-soluble sodium copper chlorophyll fit the specifications, purity 88-82%, relative extinction of 3.4-4, yield 0.8-2.05%. 5. The absorption spectrum of chlorophyll in silkworm is similar to that of mulberry leaf, while the absorption band of chlorophyll sodium is biased at two poles, between 6800-6500A° and 4760-4000A°. The wavelength-extinction curve of silkworm chlorophyll has two absorptions. Extremely, the wavelength of the chlorophyll copper nano salt - the absorption of the extinction curve is extremely great at 4070 A° and 6330 A°.
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