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自从1960年Moorhead等人建立了人外周血白细胞培养及染色体标本制备的实验方法以后,这一体外短期培养的人淋巴细胞体系,日益广泛地应用在实验医学生物学的研究中。它以分析染色体畸变的细胞遗传学方法,灵敏地揭示着各种物理、化学及病毒等因素的生物效应,大量的文献资料说明了这一实验技术所取得的广泛性成就。但是这种简便的,仅用0.3ml左右离体血进行实验,所得到的结果能否代表体内生命活动中淋巴细胞的反应,是首先必须回答的问题。本文仅就辐射诱发染色体畸变的剂量效应关系,对淋巴细胞染色体在体内外的
Since Moorhead et al. Established the experimental method of human peripheral blood leukocyte culture and chromosome preparation in 1960, this short-term cultured human lymphocyte system has been widely used in experimental medical biology research. It analyzes the biological effects of various physical, chemical and viral factors with cytogenetic analysis of chromosomal aberrations. A large body of literature illustrates the extensive achievements of this experimental technique. However, this simple, only about 0.3ml isolated blood experiments, the results obtained represent the life of lymphocytes in vivo response, is the first question that must be answered. This article only on radiation-induced chromosomal aberration dose-response relationship, the lymphocyte chromosomes in vitro and in vivo