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目的探究血管内皮生长因子(VEGF)基因多态性与动脉瘤性蛛网膜下腔出血(a SAH)的相关性。方法采用病例对照的研究方法,实验组为158例a SAH患者,均行颅脑CT检查证实为a SAH,并经脑血管造影证实是否存在颅内动脉瘤;对照组为146名健康成年人。取两组外周血,提取基因组DNA。检测VEGF2578C/A的基因型及等位基因分布频率。比较各位点基因型频率的观察值与预期值并进行卡方检验和Hardy-Weinberg平衡分析,以及VEGF基因型与a SAH的相关性。结果 1对照组VEGF的2个多态位点的基因型频率分布符合Hardy-Weinberg平衡(P>0.05)。2VEGF2578C/A基因型及等位基因分布,CC+CA基因型频率,实验组显著高于对照组(P<0.05);实验组的C等位基因频率明显高于对照组,两组比较差异有统计学意义(χ2=4.256,P=0.037)。结论 VEGF基因启动子区-2578C/A多态性可能与aSAH有关。
Objective To investigate the relationship between vascular endothelial growth factor (VEGF) gene polymorphism and aneurysmal subarachnoid hemorrhage (a SAH). Methods A case-control study was conducted in 158 patients with SAH in the experimental group. All patients underwent a brain CT scan to confirm a SAH. Cerebral angiography confirmed the presence of intracranial aneurysms. The control group was 146 healthy adults. Take two groups of peripheral blood to extract genomic DNA. The genotype and allele frequencies of VEGF2578C / A were detected. The observed and expected genotype frequencies of each locus were compared and analyzed by chi-square test and Hardy-Weinberg equilibrium analysis, and the correlation between VEGF genotype and a SAH. Results 1 The frequency distribution of genotypes of two polymorphic sites of VEGF in control group was in accordance with Hardy-Weinberg equilibrium (P> 0.05). 2VEGF2578C / A genotype and allele distribution, CC + CA genotype frequencies in the experimental group were significantly higher than those in the control group (P <0.05). The frequency of C allele in the experimental group was significantly higher than that in the control group Statistical significance (χ2 = 4.256, P = 0.037). Conclusion The -2578C / A polymorphism of VEGF gene promoter may be related to aSAH.