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目的 了解北京市海淀区城郊宿主动物携带肾综合征出血热病毒状况 ,分析宿主动物带毒相关危险因素。 方法 夜夹法捕鼠 ,计算鼠密度 ,确定鼠种构成。应用RT -PCR扩增M片段部分序列检测宿主带病毒率 ,根据检测结果应用Logistic回归分析通过SPSS( 9 0 )软件分析宿主动物感染的可能危险因素。 结果 共捕获宿主动物 2 3 8只 ,褐家鼠为优势鼠种 ,占 70 5 9%。其次为小家鼠 ,占 2 5 2 1%。RT -PCR检测宿主带病毒率 ,褐家鼠为 14 2 9% ,小家鼠为 0。就褐家鼠而言 ,尽管成鼠阳性率高于幼鼠 ,雄鼠阳性率高于雌鼠 ,但成鼠和幼鼠间、雌鼠和雄鼠间带毒率差异无显著性 (P >0 0 5 ) ,但扩增阳性鼠和扩增阴性鼠体重的差异有非常显著性 (P <0 0 0 1)。多因素分析显示宿主感染危险因素最重要的鼠个体因素尤其是体重。 结论 北京市海淀区城郊汉坦病毒感染的主要宿主动物是褐家鼠 ,采样点的鼠密度与带毒率非正相关 ,但二者可能存在更复杂的关系。
Objective To understand the status of haemorrhagic fever with renal syndrome virus in the suburbs of Haidian District, Beijing, and to analyze the risk factors associated with poisoning in host animals. Method Night clip catching mice, calculate the density of mice to determine the composition of mice. The host virus was detected by RT-PCR amplification of the M segment sequence. Logistic regression analysis was used to analyze the possible risk factors of host animal infection by SPSS software. Results A total of 238 host animals were captured, while Rattus norvegicus was the dominant species, accounting for 70 59%. Followed by Mus musculus, accounting for 2 5 2 1%. RT-PCR detection of host with virus rate, Rattus norvegicus 14 2 9%, Mus musculus 0. In the case of Rattus norvegicus, although the positive rate of adult mice was higher than that of young rats, the positive rate of male rats was higher than that of female rats, but there was no significant difference in the virulence rate between adult rats and young rats (P> 0) 0 5). However, there was a significant difference in body weight between positive and negative animals (P <0.01). Multivariate analysis showed that the most important individual risk factors for host infection were mouse individual factors, especially body weight. Conclusion The main host animal infected by Hantavirus in the suburbs of Haidian, Beijing, is Rattus norvegicus. The density of rodents in the sampled sites is not positively correlated with the virulence, but there may be more complicated relationship between the two.