在偏避山区的菇农,购买试管困难,而酒瓶几乎家家都有。为此,我们进行了酒瓶代试管生产母种试验,取得了较好效果。具体做法是:选无色透明酒瓶,洗净后沥干,将煮好的马铃薯琼脂培养基趁热分装于瓶中,每个容量为500ml 的酒瓶装培养基100ml(不要将培养基沾在瓶口上,以免污染),然后塞棉花, In avoiding mountainous mushroom farmers, difficulty buying test tubes, and almost every household has a bottle. To this end, we conducted a test tube production of the mother of the bottle test, and achieved good results. The specific practice is: Select colorless and transparent bottle, drain after washing, the cooked potato agar medium while hot in the bottle, each capacity of 500ml bottling medium 100ml (do not dip the medium In the bottle, so as to avoid pollution), then stuffed cotton,