论文部分内容阅读
目的:了解胎肾上腺线粒体代谢外源性化合物的能力和特征,方法:制备亚细胞组分,酶学检测脱甲基反应代谢产物—甲醛的含量,结果:在光谱分析和SDS-PAGE证实线粒体存在P-450的基础上,进一步证明线粒体P-450具有脱甲基功能,其脱甲基作用呈蛋白浓度(1-4 mg)和反应时间(10-30 min)依赖性增加,与底物浓度间有良好的量效关系,并与胎龄呈正比,线粒体中红霉素、苄非他明和氨基比林的脱甲基反应分别为微粒体中的89％,162％和62％。醋竹桃霉素增强肾上腺的红霉素脱甲基反应。结论:胎肾上腺线粒体有较强的脱甲基功能,提示胎儿肾上腺线粒体兼有药物代谢功能。
OBJECTIVE: To understand the ability and characteristics of fetal adrenal mitochondria to metabolize exogenous compounds. Methods: Subcellular fractions were prepared and enzymatically detected for the formaldehyde-metabolite, a demethylated metabolite. Results: The presence of mitochondria was confirmed by spectral analysis and SDS-PAGE On the basis of P-450, mitochondrial P-450 was further demonstrated to be demethylated. Demethylation of mitochondrial P-450 was dependent on protein concentration (1-4 mg) and reaction time (10-30 min) There was a good dose-response relationship and a positive correlation with gestational age. The demethylation rates of erythromycin, benfluetamine and aminopyrine in mitochondria were 89%, 162% and 62% of microsomes, respectively. Rheumatoid dexamethasone enhances erythromycin demethylation of the adrenal gland. Conclusion: The fetal adrenal mitochondria has a strong demethylation function, suggesting that the fetal adrenal mitochondria both have drug metabolism.