论文部分内容阅读
目的 :研究Bcl-2特异性抑制剂ABT-737对肿瘤相关巨噬细胞(TAMs)、乳腺癌细胞MCF-7及共培养体系上清中血管内皮生长因子(VEGF)表达的影响。方法:应用佛波酯(PMA)及白细胞介素-4(IL-4)体外诱导TAM s细胞,Transwell非接触式共培养TAMs和MCF-7细胞,四甲基偶氮唑蓝(MTT)法检测共培养后MCF-7细胞增殖情况;酶联免疫吸附法(ELISA)检测TAMs、MCF-7细胞及共培养体系上清VEGF水平。结果:应用PMA及IL-4体外诱导THP-1成为TAMs细胞,MCF-7与TAMs细胞共培养24、48 h后,细胞增殖活性较对照组分别上升了16.16%和33.99%。TAMs、MCF-7细胞及共培养体系分别加入ABT-737,VEGF含量测定值分别为(47.67±8.83)pg/mL、(108.56±10.92)pg/mL和(152.52±7.18)pg/mL,其中MCF-7细胞及共培养体系与对照组比较,差异有统计学意义(P<0.05)。结论:分泌型VEGF表达水平的增高与TAMs活化及分布有明确相关性,乳腺癌细胞与TAMs间相互作用可能通过Bcl-2-VEGF旁分泌通路实现。
Objective: To investigate the effect of ABT-737, a specific inhibitor of Bcl-2, on the expression of vascular endothelial growth factor (VEGF) in tumor-associated macrophages (TAMs), breast cancer cells MCF-7 and co-culture supernatant. Methods: TAMs cells were induced by phorbol ester (PMA) and interleukin-4 (IL-4) in vitro. Transwell non-contact co-culture of TAMs and MCF-7 cells was performed. MTT assay The proliferation of MCF-7 cells was detected by co-culture. The levels of VEGF in supernatants of TAMs, MCF-7 cells and co-culture system were detected by enzyme linked immunosorbent assay (ELISA). RESULTS: THP-1 cells were induced to TAMs cells by PMA and IL-4. After MCF-7 and TAMs cells co-cultured for 24 and 48 h, their cell proliferation activities increased by 16.16% and 33.99%, respectively. ABT-737 was added to TAMs, MCF-7 cells and co-culture system respectively, and the values of VEGF were 47.67 ± 8.83 pg / mL, 108.56 ± 10.92 pg / mL and 152.52 ± 7.18 pg / mL respectively MCF-7 cells and co-culture system compared with the control group, the difference was statistically significant (P <0.05). CONCLUSIONS: The increase of secreted VEGF expression has a clear correlation with the activation and distribution of TAMs. The interaction between breast cancer cells and TAMs may be through the paracrine Bcl-2-VEGF pathway.