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目的表达伯氏疏螺旋体鞭毛蛋白特异性区段，探讨其作为诊断抗原的可行性。方法用ＰＣＲ方法获取与其它种属螺旋体编码鞭毛蛋白的基因序列同源性较低的第４０９－７８６ｂｐ区段，经ＤＮＡ序列测定证实后，经克隆、转化，在大肠杆菌ＢＬ２１（ＤＥ３）中表达，并做Ｗｅｓｔｅｒｎ－ｂｏｌｔ分析。结果重组蛋白在宿主菌内表达高效、稳定，Ｗｅｓｔｅｒｎ－ｂｌｏｔ示与抗鞭毛蛋白的单克隆抗体有较好的免疫反应性。结论以重组鞭毛蛋白的特异性区段作为诊断抗原具有可行性。
Objective To express the specific region of Borrelia burgdorferi and to explore its feasibility as a diagnostic antigen. Methods The fragment of 409-786 bp with low homology with other species of spirochetes coding for flagellin was obtained by PCR. After confirmed by DNA sequencing, it was cloned, transformed and expressed in E. coli BL21 (DE3) , And do Western-bolt analysis. Results The recombinant protein was expressed efficiently and stably in the host bacteria. Western-blot showed that the recombinant protein had good immunoreactivity with the anti-flagellin monoclonal antibody. Conclusion It is feasible to use the specific segment of recombinant flagellin as diagnostic antigen.