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选择２７ 个识别序列为４ ｎｔ 的限制性内切酶， 通过计算机对最新获得的１９ 个ＤＱＡ１和３５ 个ＤＱＢ１ 等位基因第２ 外显子中的一段序列进行模拟酶切分析， 根据酶切格局数目及各格局所代表的等位基因数的均衡性， 确定酶的优先次序， 在此基础上进行ＰＣＲＲＦＬＰ模拟分析， 确定最佳的酶组合． 结果表明， 分别采用４ 个和６ 个酶的组合， 可有效鉴定ＤＱＡ１ 和ＤＱＢ１ 等位基因． 同时通过比较分析ＤＱＢ１ 各等位基因核苷酸位点的变异系数， 发现ＤＱＢ１ 不同血清型间等位基因第２ 外显子核苷酸序列变化有一定规律． 研究为建立一个简捷准确的ＨＬＡ 分型方法提供了新的理论基础．
27 restriction endonucleases with a recognition sequence of 4 nt were selected and the sequences of the newly obtained exons 2 of 19 DQA1 and 35 DQB1 alleles were mimicked by computer. According to the restriction pattern Number and the pattern of the number of alleles represented by the balance to determine the priorities of the enzyme on the basis of PCR RFLP simulation analysis to determine the best combination of enzymes. The results showed that the combination of 4 and 6 enzymes, respectively, can effectively identify the DQA1 and DQB1 alleles. At the same time, by comparing and analyzing the coefficient of variation of nucleotide of each allele of DQB1, we found that the nucleotide sequence of exon 2 of different serotypes of DQB1 have certain rules. The research provides a new theoretical basis for establishing a simple and accurate HLA typing method.