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本试验以薄荷试管苗叶片为外植体,研究了激素、光照、褐变抑制剂对不定芽诱导的影响,进而筛选出合适的生根培养基。研究结果表明:薄荷叶片最佳不定芽诱导的培养基为MS+1.4 mg/L TDZ+0.2 mg/L NAA+30 g/L蔗糖+5.5 g/L琼脂,诱导率达27.78%;暗培养20 d后再转接一次,叶片不定芽诱导率提高至64.29%,褐化率低至11.90%;最佳生根培养基为MS+0.1 mg/L NAA+20 g/L蔗糖+5.5 g/L琼脂,生根率达97.50%;再生苗经生根、炼苗后移栽,植株成活率达100%。本试验建立了薄荷叶片离体再生体系,为薄荷无菌苗生产和遗传转化研究提供了技术支撑。
In this experiment, the leaves of peppermint plantlets were used as explants to study the effects of hormones, light and browning inhibitors on the induction of adventitious buds, and then the suitable rooting medium was screened out. The results showed that the optimum medium for induction of mint leaves was MS + 1.4 mg / L TDZ + 0.2 mg / L NAA + 30 g / L sucrose + 5.5 g / L agar with the induction rate of 27.78% d and then transferred again, the induction rate of adventitious buds increased to 64.29% and the browning rate was as low as 11.90%. The best rooting medium was MS + 0.1 mg / L NAA + 20 g / L sucrose + 5.5 g / L agar , And the rooting rate reached 97.50%. The regenerated seedlings were rooted, harvested and transplanted. The survival rate of the plants reached 100%. In this experiment, the regeneration system of mint leaves in vitro was established, which provided technical support for the production and genetic transformation of mint aseptic seedlings.