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寻找最适合的用于防治骨质疏松症新药筛选的成骨细胞系。方法：采用不同来源组织培养成骨细胞，分别加入已知对成骨细胞有促骨形成作用的地塞米松和氢化可的松，通过ＶｏｎＫｏｓａ染色，定量表示出骨形成，比较不同组织培养的成骨细胞用于药物筛选的优点和缺点。结果：ＭＣ３Ｔ３Ｅ１细胞最易获得，其次是大鼠胎儿成骨细胞；小鼠成骨细胞培养周期长，成活率低；大鼠大腿骨成骨细胞稳定性不好，培养方法还不成熟；成熟大鼠成骨细胞培养方法成熟。结论：成熟大鼠成骨细胞是用于骨质疏松症相关的骨形成研究和其药物筛选最适宜的成骨细胞系。
Find the most suitable osteoporosis cell line for the prevention and treatment of osteoporosis. Methods: Osteoblasts were cultured from different tissues. Dexamethasone and hydrocortisone, which are known to promote bone formation, were added into osteoblasts. VonKosa staining was used to quantitatively show bone formation. The advantages and disadvantages of bone cells for drug screening. RESULTS: MC3T3E1 cells were the most available, followed by rat fetal osteoblasts. Osteoblasts grew slowly and had low survival rate. The stability of osteoblasts in the femur was not good and the method of culture was not mature. Mature rat osteoblast culture method is mature. Conclusion: Adult rat osteoblasts are the most suitable osteoblast cell line for osteoporosis-related bone formation and drug screening.