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目的探讨铜绿假单胞菌生物膜致病性。方法 60只豚鼠随机分为生物膜组、浮游菌组和对照组,行气管切开术后分别植入生物膜细菌、浮游态细菌及生理盐水包被的硅胶管,15 d后收集支气管肺泡灌洗液(BALF)、支气管及肺组织,测量活菌数,分离细菌并测定生物膜形成能力,观察组织形态改变。结果生物膜组豚鼠下呼吸道慢性感染发生率为66.7%,明显高于浮游菌组(P<0.01);生物膜组豚鼠支气管及肺组织可见肺泡腔内纤维渗出,间隔炎性细胞浸润,纤毛上皮增生,黏膜下淋巴细胞浸润;生物膜组豚鼠BALF及肺组织中细菌总数分别为(7.45±0.23)×103 cfu/mL和(6.78±0.36)×103 cfu/g,并分离出能够形成生物膜的铜绿假单胞菌。结论铜绿假单胞菌生物膜是下呼吸道慢性感染的高危因素之一。
Objective To investigate the pathogenicity of biofilms of Pseudomonas aeruginosa. Methods Sixty guinea pigs were randomly divided into biofilm group, planktonic group and control group. After tracheotomy, biofilm bacteria, floating bacteria and saline-coated silicone tube were implanted. After 15 days, bronchoalveolar lavage fluid Lotion (BALF), bronchus and lung tissue, measuring viable cells, isolating bacteria and measuring biofilm formation ability, and observing the change of tissue morphology. Results In the biofilm group, the incidence of chronic lower respiratory tract infection in guinea pigs was 66.7%, which was significantly higher than that in the planktonic bacteria group (P <0.01). In the biofilm group, bronchoalveolar and lung tissue showed alveolar fiber exudation, inflammatory cell infiltration and cilia (7.45 ± 0.23) × 103 cfu / mL and (6.78 ± 0.36) × 103 cfu / g respectively in BALF and lung tissue of biofilm group, and the number of bacteria capable of forming biological Membranous Pseudomonas aeruginosa. Conclusion Pseudomonas aeruginosa biofilm is one of the risk factors of chronic lower respiratory tract infection.