论文部分内容阅读
通过分析细菌细胞的结构特征,将细菌菌体的多波长散射分为外部结构散射和内部结构散射两个部分,建立了细菌菌体前向散射光谱解释模型。利用该模型对大肠杆菌400~900nm波段的前向散射光进行了快速解析,得到了大肠杆菌外部结构、内部结构的平均粒径大小及两结构占细菌体前向散射的比例;基于单细胞的散射光密度与整体细菌悬浮液光密度之间的关系可以快速检测出细菌的浓度。多次细菌浓度测量结果之间的最大差异为1.83%,且与平板法相比较,测量结果在同一量级,相对误差为3.44%。对不同生长时期的大肠杆菌和肺炎克雷伯菌进行了光谱解析,得到了两种细菌浓度及菌体大小随时间的变化曲线。研究结果不仅为细菌微生物生长过程的科学研究提供了一种快捷方法,而且为水体细菌微生物的快速检测与预警提供了技术手段。
By analyzing the structural characteristics of bacterial cells, the multiwavelength scattering of bacterial cells was divided into two parts: external structure scattering and internal structure scattering, and the model of bacterial cell forward scattering was established. The forward scattering light of Escherichia coli in the band of 400-900 nm was rapidly analyzed by this model. The average size of the outer and inner structures of Escherichia coli and the ratio of the two structures to the front scatter of the bacterial cells were obtained. Based on single cell The relationship between the scattered optical density and the overall bacterial suspension optical density allows rapid detection of bacterial concentration. The maximum difference between multiple bacterial concentration measurements was 1.83%, and compared with the plate method, the measurement results were on the same order with a relative error of 3.44%. Spectra of Escherichia coli and Klebsiella pneumoniae at different growth stages were analyzed by spectroscopy, and the curves of the two bacterial concentrations and cell size with time were obtained. The results not only provide a quick method for the scientific research of bacterial microbial growth process, but also provide a technical measure for the rapid detection and early warning of bacterial microbes in water.