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建立了反相高效液相色谱法同时测定银黄含片中的黄芩苷、绿原酸和芦丁。采用HypersilODSC18柱(200mm×4.6mm,粒径5μm),流动相A:甲醇-水-乙酸(10∶88∶2)和流动相B:甲醇-水-乙酸(88∶10∶2),梯度洗脱程序0min(A∶B/97∶3)-10min(A∶B/73∶27)-25min(A∶B/20∶80)-36min(A∶B/20∶80);流速1.0mL/min;检测波长为327nm和280nm;柱温25℃。黄芩苷线性范围为0.051—0.512μg/mL,r=0.9990,样品的平均加标回收率为98.23%,(RSD为2.13%);绿原酸线性范围为0.059—0.592μg/mL,r=0.9996,样品的平均加标回收率为97.61%,(RSD为2.13%);芦丁线性范围为0.087—0.868μg/mL,r=0.999,样品的平均加标回收率为98.56%,(RSD为1.13%),该方法专属性强,结果稳定,重现性好,能有效地控制药品的主成分含量,具有较强的实用价值。
A reversed-phase high performance liquid chromatographic method was established for simultaneous determination of baicalin, chlorogenic acid and rutin in Yinhuang tablets. Using a Hypersil ODSC18 column (200 mm x 4.6 mm, particle size 5 μm), mobile phase A: methanol-water-acetic acid (10:88:2) and mobile phase B: methanol-water-acetic acid (88:10:2), gradient washing Deblocking 0min(A:B/97:3)-10min(A:B/73:27)-25min(A:B/20:80)-36min(A:B/20:80); Flow rate 1.0mL/ Min; detection wavelength is 327nm and 280nm; column temperature 25°C. The linear range of baicalin was 0.051-0.512 μg/mL, r=0.9990. The average recovery of the sample was 98.23% (RSD was 2.13%). The linear range of chlorogenic acid was 0.059-0.592 μg/mL, r=0.9996. The average recovery rate of the sample was 97.61% (RSD was 2.13%); the linear range of rutin was 0.087-0.868 μg/mL, r=0.999, the average recovery rate of the sample was 98.56%, (RSD was 1.13. %)) This method has strong specificity, stable results, good reproducibility, and can effectively control the main component content of drugs. It has strong practical value.