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目的:研究蛇葡萄素钠(AMP-Na)对人肝癌HepG2细胞株的X射线增敏作用,并探讨其作用机制。方法:应用MTT法和集落形成法测定AMP-Na对HepG2细胞的X射线增敏作用,单因素方差分析确定增敏作用,单击多靶模型曲线拟合计算增敏比;流式细胞术检测其对细胞周期和凋亡的影响。结果:MTT检测结果表明,AMP-Na能显著抑制HepG2细胞的增殖,并呈时间和剂量依赖效应;对X射线增敏作用方差分析表明,AMP-Na能增加X射线对肝癌HepG2细胞的敏感性,最佳增敏剂量为12.5～25μg/mL。集落形成法检测结果表明,应用增敏剂量的AMP-Na后,D0、Dq和N值减小,增敏比为1.94。流式细胞仪检测表明20μg/mL AMP-Na和X射线合用后出现明显的诱导细胞凋亡作用,细胞周期主要表现为S期阻滞,G2/M期细胞比例明显减少,表明联合应用具有增强效应,但细胞周期作用点仍以S期为主。结论:AMP-Na能增强肝癌HepG2细胞对X射线的敏感性,最佳增敏剂量为12.5～25μg/mL,其机理可能通过诱导细胞凋亡、调控细胞周期停滞于S期发挥作用。
Objective: To investigate the X-ray sensitization of HepG2 cells to HepG2 cells and to explore its mechanism. Methods: The sensitization effect of AMP-Na to HepG2 cells was determined by MTT assay and colony formation assay. The sensitization effect was determined by one-way ANOVA and the sensitization ratio was calculated by curve fitting of multi-target model. Flow cytometry Its effect on cell cycle and apoptosis. Results: The results of MTT assay showed that AMP-Na significantly inhibited the proliferation of HepG2 cells in a time and dose-dependent manner. The analysis of variance of X-ray sensitization showed that AMP-Na increased the sensitivity of X-ray to HepG2 cells , The best dose of sensitization is 12.5 ~ 25μg / mL. Colony formation assay results showed that the D0, Dq and N values decreased with increasing sensitizing dose of AMP-Na, and the sensitization ratio was 1.94. Flow cytometry showed that 20μg / mL AMP-Na and X-ray combined with significant induction of apoptosis, the cell cycle was mainly S phase arrest, G2 / M phase cells decreased significantly, indicating a combination of enhanced Effect, but the role of cell cycle is still the main S phase. Conclusion: AMP-Na can enhance the sensitivity of HepG2 cells to X-ray. The optimal dose of sensitizing agent is 12.5-25 μg / mL. The mechanism may be through the induction of apoptosis and the regulation of cell cycle arrest in S phase.