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设不同浓度赖氨酸(L)加苏氨酸(T)的培养基,将opaqure-2等32个玉米自交系种子的胚接种在培养基上发芽,同时测定种子中游离氨基酸和总氨基酸含量,检测胚生长对L+T的抗性与种子中赖氨酸含量关系,筛选鉴定玉米种子中高赖氨酸含量的有效方法。结果表明,opaqure-2对L+T有较强抗性,子粒中有较高含量游离赖氨酸;普通玉米自交系抗L+T能力较弱,种子中游离氨基酸含量较低;在旅大红骨类群中检测到对L+T有较强抗性的基因型,子粒中有较高含量的赖氨酸和其他氨基酸。玉米中存在典型的天冬族氨基酸生物合成的反馈抑制代谢途径,试管L+T胁迫方法可用于筛选和鉴定玉米高赖氨酸基因型。
The medium containing lysine (L) and threonine (T) at different concentrations was used to germinate the embryos of 32 maize inbred lines, such as opaqure-2. The free amino acids and total amino acids Content, detection of embryo growth resistance to L + T lysine content in the seed, screening and identification of corn high lysine content of the effective method. The results showed that opaqure-2 had stronger resistance to L + T and higher content of free lysine in the grain. The common maize inbred lines had weaker resistance to L + T and lower content of free amino acids in the seeds. The genotypes with stronger resistance to L + T were detected in the big red bone group, and the lysine and other amino acids were higher in the granule. In corn, there are typical metabolic pathway of amino acid biosynthesis in asparagus and the L + T stress can be used to screen and identify the high lysine genotype in maize.