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目的:研究白花前胡甲素(Pd-Ia)对人大脑皮层神经元ATP敏感钾通道的作用。方法:应用膜片箱全细胞记录技术,采用累积给药方式。箝制电压-40mV,指令电压-30到+100mV,时程600ms。结果:Pd-Ia以浓度依赖方式激活ATP敏感钾通道。当用含不同浓度的(0.01,0.01,0.1和1μmol/L)Pd-Ia细胞外液灌流时,电流值分别从给药前的(0.9±0.4)nA增大到给药后的(1.0±0.4)nA,(1.1±0.4)nA,(1.2±0.4)nA和(1.3±0.4)nA(P<0.01,n=5)。然后用含ATP敏感钾通道的特异抑制剂格列苯肥(10μmol/L)的细胞外液冲洗,电流减小至(0.90±0.37)nA(P<0.01,n=5)。结论:Pd-Ia可开放ATP敏感钾通道,是一种钾通道开放剂。
OBJECTIVE: To study the effect of Pd-Ia on ATP-sensitive potassium channels in human cerebral cortex neurons. Methods: The whole-cell recording technique was applied to the diaphragm box and the cumulative administration method was used. Clamp voltage -40mV, command voltage -30 to +100mV, duration 600ms. Results: Pd-Ia activated ATP-sensitive potassium channels in a concentration-dependent manner. When perfused with Pd-Ia extracellular fluids containing different concentrations (0.01, 0.01, 0.1 and 1 μmol/L), the current values increased from (0.9±0.4) nA before drug administration to (1.0±) after drug administration. 0.4) nA, (1.1±0.4) nA, (1.2±0.4) nA and (1.3±0.4) nA (P<0.01, n=5). Then, the extracellular fluid containing a specific inhibitor of ATP-sensitive potassium channels, glyphenone (10 μmol/L), was flushed and the current was reduced to (0.90±0.37) nA (P<0.01, n=5). CONCLUSION: Pd-Ia can open ATP-sensitive potassium channels and is a potassium channel opener.