论文部分内容阅读
目的探讨慢性体外反搏对高脂饲养猪动脉内皮细胞基因表达的影响。方法选雄性乳猪22头用高脂喂养建立动脉粥样硬化模型,对模型猪进行36h体外反搏治疗。对冠状动脉作透射电镜检查。对腹主动脉作苏丹III染色,分析脂质浸润阳性率。用基因芯片分析猪的胸主动脉内皮细胞基因的表达。结果透射电镜显示,高脂组冠状动脉内皮细胞下层可见巨噬细胞和分化的泡沫细胞,正常组、高脂加反搏组内皮细胞下层完整。正常组和高脂加反搏组与高脂组腹主动脉苏丹III染色阳性率有显著差异(P<0.05)。芯片检测显示,与正常组相比,高脂组整合素β1、CTGF上调;与高脂组相比,反搏组整合素β1、CTGF,和VCAM-1下调,eNOS上调。结论慢性体外反搏可以下调动脉内皮细胞整合素β1、CTGF和VCAM-1基因的表达,降低动脉內皮细胞对脂质的摄取,改善动脉内皮功能,起到抗动脉粥样硬化的作用。
Objective To investigate the effects of chronic exogenous counterpulsation on the gene expression of high-fat-fed porcine arterial endothelial cells. Methods Twenty-two male suckling pigs were fed with high-fat diet to establish atherosclerosis model and 36-hour external counterpulsation treatment on model pigs. The coronary artery for transmission electron microscopy. Sudan III staining was performed on the abdominal aorta, and the positive rate of lipid infiltration was analyzed. Analysis of Porcine Thoracic Aortic Endothelial Cell Gene Expression Using Gene Chips. Results Transmission electron microscopy revealed macrophages and differentiated foam cells in the lower layer of coronary artery endothelial cells in the hyperlipidemic group. In the normal group, the lower layer of the endothelial cells in the hyperlipidemic and counterpulsation group was intact. The positive rate of Sudan III staining of abdominal aorta in normal group, hyperlipidemic group and hyperlipidemic group were significantly different (P <0.05). Compared with normal group, the expression of integrinβ1 and CTGF in high-fat group was higher than that in normal group. Compared with high-fat group, the levels of integrinβ1, CTGF, VCAM-1 and eNOS were up-regulated. Conclusion Chronic EECP can down-regulate the expression of integrin β1, CTGF and VCAM-1 in arterial endothelial cells, decrease the uptake of lipids by endothelial cells, improve the endothelial function of arteries and play an anti-atherogenic role.