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目的:探讨苦参碱(Mat)对大鼠原位肝移植冷缺血再灌注损伤的肝窦内皮细胞的保护作用及其机制。方法:应用大鼠原位肝移植模型,供肝保存5小时。126只大鼠随机分为对照组、Mat 40mg/kg治疗组、Mat 80mg/kg治疗组和假手术组,分别检测移植术后1、2、4小时血透明质酸及肝组织细胞间粘附分子-1的含量,并观察肝血窦内皮细胞形态学的改变。结果:与对照组比较,治疗组术后各时点的透明质酸和细胞间粘附分子-1表达均显著降低,肝血窦内皮细胞形态变化也有明显改善。结论:苦参碱在大鼠原位肝移植冷缺血再灌注中保护肝血窦内皮细胞免受损伤。
Objective: To investigate the protective effect of matrine on liver sinusoidal endothelial cells after orthotopic liver transplantation in rats with cold ischemia-reperfusion injury. METHODS: Rat orthotopic liver transplantation model was used and the donor liver was preserved for 5 hours. 126 rats were randomly divided into control group, Mat 40 mg/kg treatment group, Mat 80 mg/kg treatment group and sham operation group. Blood hyaluronic acid and hepatic tissue cell adhesion were measured at 1, 2, and 4 hours after transplantation. The content of molecule-1 was observed, and the morphological changes of hepatic sinusoidal endothelial cells were observed. RESULTS: Compared with the control group, the expression of hyaluronic acid and intercellular adhesion molecule-1 was significantly decreased at each time point in the treatment group, and the morphological changes of hepatic sinusoidal endothelial cells were also significantly improved. CONCLUSION: Matrine protects hepatic sinusoidal endothelial cells from injury during cold ischemia-reperfusion in orthotopic liver transplantation in rats.