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目的:探讨转化生长因子β激活酶1(TAK1)在结肠癌组中的表达与临床意义。方法:收集141例结肠癌患者手术标本,用免疫组织化学方法检测TAK1蛋白在结肠癌及癌旁正常组织中的表达,并分析其表达与患者临床病理因素及预后的关系,同时检测结肠癌组织中K-ras基因突变情况,分析TAK1表达与K-ras基因突变的关系。结果:TAK1在结肠癌组织中的阳性表达率明显高于癌旁正常组织(68.8%vs.16.3%,P<0.05);TAK1的阳性表达与Dukes分期、肿瘤分化程度和淋巴结转移有关(P<0.05);TAK1阳性表达患者的5年生存率明显低于低表达的患者(P<0.05);TAK1阳性表达的结肠癌组织K-ras基因的突变率明显高于TAK1阴性表达的结肠癌组织(52.6%vs.13.6%,P<0.05)。结论:TAK1可能参与了结肠癌的恶性进展,且TAK1的表达可能与K-ras基因突变密切相关。
Objective: To investigate the expression and clinical significance of transforming growth factor-beta-1 (TAK1) in colon cancer. Methods: The specimens of 141 patients with colon cancer were collected. The expression of TAK1 protein in colon cancer tissues and adjacent normal tissues was detected by immunohistochemistry. The relationship between the expression of TAK1 protein and the clinicopathological factors and prognosis was analyzed. Meanwhile, In K-ras gene mutations, analysis of TAK1 expression and K-ras gene mutations. Results: The positive expression rate of TAK1 in colon cancer tissues was significantly higher than that in adjacent normal tissues (68.8% vs.16.3%, P <0.05). The positive expression of TAK1 was correlated with Dukes stage, tumor differentiation and lymph node metastasis (P < 0.05). The 5-year survival rate of patients with TAK1 positive expression was significantly lower than that of patients with low expression (P <0.05). The mutation rate of K-ras gene in TAK1 positive colon cancer tissues was significantly higher than that of TAK1 negative colon cancer tissues 52.6% vs.13.6%, P <0.05). Conclusion: TAK1 may be involved in the malignant progression of colon cancer, and the expression of TAK1 may be closely related to the mutation of K-ras gene.