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目的:观察脾胃湿热证大鼠醛固酮(ALD)和抗利尿激素(ADH)改变情况,探讨三仁汤的作用机理。方法:将50只雄性Wistar大鼠随机分为正常组、模型组、三仁汤(高、中、低剂量)组,通过复合因素(外部湿热环境加肥甘饮食加病原微生物)造模,采用放射免疫法检测大鼠血清ALD和血浆ADH的水平。结果:模型组大鼠血清ALD及血浆ADH升高明显,三仁汤(高、中、低剂量)组可以降低血清ALD和血浆ADH水平(P<0.05,P<0.01)。结论:醛固酮、抗利尿激素升高,可能是脾胃湿热证发生的机理之一;三仁汤调节ALD、ADH的释放水平,可能是其清热祛湿的作用机制之一。
OBJECTIVE: To observe the changes of aldosterone (ALD) and antidiuretic hormone (ADH) in rats with spleen-stomach damp-heat syndrome and to investigate the mechanism of action of Sanren decoction. METHODS: Fifty male Wistar rats were randomly divided into the normal group, the model group, and the Sanren Tang (high, middle, and low dose) groups, and they were modeled by compound factors (external moist heat environment plus fat and sweet diet and pathogenic microorganisms). Radioimmunoassay was used to detect serum ALD and plasma ADH levels in rats. RESULTS: Serum ALD and plasma ADH increased significantly in the model group, and serum ALD and plasma ADH levels were decreased in the Sanren Tang (high, medium, and low dose) group (P<0.05, P<0.01). Conclusion: The increase of aldosterone and antidiuretic hormone may be one of the mechanisms of spleen-stomach dampness-heat syndrome. Sanren Decoction can regulate the release of ALD and ADH, which may be one of the mechanisms of heat-clearing and dampness.