用成年大白鼠20只,将国产樱草黄和伊凡思兰二种萤光染料分别注射至尾壳核头部的不同部位。实验结果表明,两种萤光染料均可用作萤光逆行性双标记研究。本文叙述了萤光逆行性双标记技术的操作步骤和体会,并介绍了一种简便而不影响萤光标记的萤光逆行性标记和儿茶酚胺神经元萤光组化联合法。 Twenty adult rats were used to inject two kinds of fluorescent dyes, Primrose Yellow and Evans Blue, into different parts of the caudate putamen. The experimental results show that both fluorescent dyes can be used as fluorescent double retrograde labeling studies. This article describes the steps and experiences of fluorescent retrograde double labeling technology and introduces a fluorescent retroreflective labeling method that is simple and does not affect fluorescent labeling and a cofatellin neuronal fluorimetric combination method.