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目的 观察磷脂酰肌醇 - 3激酶 /蛋白激酶通路 (PI3K/AKT)在不同危险分组的白血病细胞中磷酸化激活的程度 ,初步了解急性白血病细胞PI3K/AKT激活与急淋白血病危险分组的关系。方法 使用蛋白印记分析法(WesternBlot)检测正常健康儿童、初发白血病患儿、缓解后患儿以及难治性白血病患儿白血病骨髓细胞中PI3K/AKT磷酸化激活程度。结果 正常健康儿童骨髓单个核细胞中无PI3K/AKT磷酸化激活 ,10例初发患儿中 3例、10例缓解后患儿 1例有PI3K/AKT磷酸化激活 ,6例难治患儿中 4例有PI3K磷酸化激活。结论 PI - 3K/AKT信号传导通路激活与否在淋巴细胞白血病危险分组和疾病转归的生物学过程中可能起着较重要作用 ,需进一步深入研究。
Objective To investigate the phosphorylation and activation of PI3K / AKT in leukemia cells of different risk groups and to understand the relationship between activation of PI3K / AKT and the risk of acute leukemia in acute leukemia cells. Methods Western blotting was used to detect the phosphorylation of PI3K / AKT in leukemia bone marrow cells of children with normal, children with primary leukemia, children with remission and children with refractory leukemia. Results There was no phosphorylation of PI3K / AKT in the bone marrow mononuclear cells of normal healthy children, 3 of 10 infants with initial onset and 1 of 10 infants with post-remission phosphorylation activation of PI3K / AKT. Among 6 refractory children Four cases had PI3K phosphorylation. Conclusion The activation of PI - 3K / AKT signal transduction pathway may play an important role in the biological process of lymphocytic leukemia risk grouping and disease prognosis, which needs further study.