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目的:探讨大黄酸(Rhein)对高糖和血管紧张素Ⅱ(angiotensin,AngⅡ)诱导的大鼠近端肾小管上皮细胞肥大的影响。方法:麻醉下,无菌分离大鼠单根近球小管并培养,以免疫细胞化学方法鉴定体外培养的肾小管上皮细胞。在高糖(30 mmol/L)环境下用AngⅡ(10-7mol/L)刺激肾小管上皮细胞肥大。与此同时,加入不同浓度的大黄酸(30、15、5 mg/L),作用72 h后检测细胞体积、3H-亮氨酸掺入量以及蛋白质含量以观察细胞肥大的变化。结果:高糖(30 mmol/L)+AngⅡ(10-7mol/L)培养72 h导致细胞体积显著增大,3H-亮氨酸掺入量及细胞内蛋白质含量显著增加。加入大黄酸处理72 h后,大黄酸30 mg/L可显著降低高糖和AngⅡ所致的细胞体积增大、3H-亮氨酸掺入量及细胞内蛋白质含量的升高。大黄酸15 mg/L降低了3H-亮氨酸掺入量及细胞内蛋白质含量,而对细胞体积无显著抑制作用。大黄酸5 mg/L可降低细胞内蛋白质含量,而对细胞体积及3H-亮氨酸掺入量无显著抑制作用。结论:大黄酸能抑制高糖和AngⅡ诱导的肾小管上皮细胞肥大。
AIM: To investigate the effect of rhein on proximal tubular epithelial cell hypertrophy induced by high glucose and Angiotensin (AngⅡ) in rats. Methods: Under anesthesia, single proximal tubule of a rat was aseptically isolated and cultured. Immunocytochemistry was used to identify the renal tubular epithelial cells in vitro. In high glucose (30 mmol / L) environment with Ang Ⅱ (10-7mol / L) to stimulate renal tubular epithelial cell hypertrophy. At the same time, different concentrations of rhein (30, 15, 5 mg / L) were added and the cell volume, 3H-leucine incorporation and protein content were measured 72 hours later to observe the changes of cell hypertrophy. RESULTS: After cultured with high glucose (30 mmol / L) and AngⅡ (10-7 mol / L) for 72 h, the cell volume was significantly increased, and 3H-leucine incorporation and intracellular protein content were significantly increased. After 72 h treatment with rhein, rhein at 30 mg / L significantly decreased the volume of cells induced by high glucose and AngⅡ, the incorporation of 3H-leucine and the increase of intracellular protein content. Rhein acid 15 mg / L reduced 3H-leucine incorporation and intracellular protein content, but had no significant inhibitory effect on cell volume. Rhein acid 5 mg / L can reduce the intracellular protein content, but the cell volume and 3H-leucine incorporation no significant inhibitory effect. Conclusion: Rhein can inhibit the hypertrophy of renal tubular epithelial cells induced by high glucose and Ang Ⅱ.