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1988年以来,我们在青岛地区对部分腹泻人群进行了隐孢子虫感染的调查,报道如下。一、材料和方法以青岛市内及郊区医院肠道门诊就医的腹泻患者为调查对象,作一般情况登记并收集粪便标本,带回实验室,除做常见肠道寄生虫检查外,用金胺——酚染色,在荧光显微镜下筛检隐孢子虫卵囊,并记录卵囊感染度。阳性或可疑标本,再用改良抗酸染色法染色,光学显微镜下确诊。对卵囊阳性者进行随访,做流行病学调查,并再次复查粪便,仍有卵囊,即给予口服大蒜素胶囊。并收集阳性者家庭成员及与之密切接触者粪便进行检查。用光镜及电镜对卵囊形态进行观察。
Since 1988, we conducted a survey of cryptosporidiosis in some diarrhea population in Qingdao. The report is as follows. First, the material and methods to diarrhea patients admitted to the intestine clinic in Qingdao City and suburban hospitals as the survey object, for general registration and collection of stool specimens, back to the laboratory, in addition to do common intestinal parasites, with gold amine - Phenol staining, screening Cryptosporidium oocysts under a fluorescence microscope, and recording the degree of oocyst infection. Positive or suspicious specimens, then stained with modified acid-fast staining, confirmed by light microscopy. Follow-up of oocyst-positive, do epidemiological investigation, and re-examine the stool, there are still oocysts, that is given oral allicin capsules. And collected positive family members and their close contact with excrement inspection. The morphology of oocysts was observed with light microscope and electron microscope.