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应用巢式ＰＣＲ结合碱性磷酸酶标记的ＡＳＯ探针杂交技术和ＤＮＡ测序技术对１２７例乙肝病毒（ＨＢＶ）感染后的血清标本进行了ＨＢＶ基因前Ｃ区突变的检测。结果发现：５３例为突变株感染，其中３２例为无症状携带者；１５例为慢性肝炎患者；肝硬化和肝癌患者各３例。在１２７例标本中发现４种新生突变：Ｉ１０Ｎ、Ｃ１２Ｗ、Ｃ１４Ｓ和Ｖ１７Ｆ，和４种不同数目核苷酸的插入片段。本研究认为ＨＢＶ基因前Ｃ区突变不一定导致病情加重。碱性磷酸酶标记的ＡＳＯ杂交技术安全可靠，特别适用于临床多样本的突变检测。
A total of 127 serum samples from patients with hepatitis B virus (HBV) infection were detected by nested PCR and alkaline phosphatase labeled ASO probe hybridization and DNA sequencing. The results showed that: 53 cases were infected with the mutant, of which 32 cases were asymptomatic carriers; 15 cases of patients with chronic hepatitis; liver cirrhosis and liver cancer in 3 cases. Four newborn mutations were found in 127 specimens: I10N, C12W, C14S and V17F, and insertions of four different numbers of nucleotides. This study suggests that mutations in the pre-C region of the HBV gene may not necessarily result in aggravation. Alkaline phosphatase-labeled ASO hybridization technology is safe and reliable, especially for clinical multi-sample mutation detection.