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目的 探讨超抗原葡萄球菌肠毒素A(SEA)和葡萄球菌肠毒素B (SEB)对HBsAg诱导小鼠产生抗 -HBs抗体水平及阳转率作用。方法 BABL/C小鼠 60只 ,随机分为 5组。组 1小鼠在左腹股沟皮下注射重组HBsAg 4ug ,每周 1次 ,连续 3周。余 4组HBsAg注射情况同组 1,但同时给予SEA或SEB。组 2注射HBsAg 1d后 ,在对侧腹股沟皮下注射SEA 2 5 μg、组 3注射SEB 2 5 μg ;组4第 2周注射HBsAg同时对侧腹股沟皮下注射SEA 2 5 μg、组 5注射SEB 2 5 μg。每周取血一次 ,采用ELISA方法检测小鼠血清抗 -HBs抗体水平。结果 组 1第 3周抗 -HBs阳转率为 41 7% ,抗 -HBs抗体水平 ( 0 75 8± 0 12 6) ;组 2、组 4第 3周抗 -HBs阳转率均为 10 0 % ,抗 -HBs抗体水平与组 1相比均差异显著 (P <0 0 5 )。而组 3、组 5第 3周抗 -HBs抗体水平与组 1相比均无显著性差异(P >0 0 5 )。结论 SEA可显著提高HBsAg免疫后的小鼠抗 -HBs抗体水平及阳转率 ,对HBsAg诱导小鼠体液免疫有明显的增强作用。
Objective To investigate the effect of superantigen staphylococcal enterotoxin A (SEA) and staphylococcal enterotoxin B (SEB) on the anti-HBs antibody production and the positive conversion rate in HBsAg-induced mice. Methods Sixty BABL / C mice were randomly divided into five groups. Group 1 mice were injected subcutaneously in the left groin with recombinant HBsAg 4ug once a week for 3 weeks. The other 4 groups of HBsAg injection with the same group 1, but given SEA or SEB. In group 2, 1 g of HBsAg was injected subcutaneously in the contralateral groin, and SEB 2 was injected subcutaneously into the contralateral groin. SEB 2 5 μg was injected into group 3. In group 2, HBsAg was injected into the lateral groin at the same time. μg. Blood was taken once a week, and the level of anti-HBs antibody in serum was detected by ELISA. Results The positive rate of anti-HBs was 41 7% and the level of anti-HBs antibodies (0 75 8 ± 0 12 6) in the third week in group 1. The positive rate of anti-HBs in groups 2 and 4 was 10 %, Anti-HBs antibody levels were significantly different from group 1 (P <0 05). However, there was no significant difference in anti-HBs antibody level between group 3 and group 3 at the third week (P> 0.05). Conclusion SEA can significantly increase the anti-HBs antibody level and positive rate of mice after HBsAg immunization, and significantly enhance the humoral immunity of mice induced by HBsAg.