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目的：探讨增殖细胞核抗原（PCNA）和调亡抑制基因bcl-2产物在宫颈鳞状细胞癌组织中的表达规律以及与宫颈鳞状细胞癌的关系．方法：应用SP（链霉抗生物素蛋白一过氧化物酶）免疫组织化学方法，检测43例宫颈鳞状细胞癌（Ⅰ级10例，Ⅱ级22例，Ⅲ级11例），24例宫颈不典型增生，16例正常宫颈组织中PCNA，bcl-2基因产物的表达．结果：PCNA在子宫颈鳞状细胞癌组织中呈明显阳性表达，定位于宫颈鳞癌细胞核，PCNA在宫颈鳞癌Ⅰ，Ⅱ，Ⅲ各级中的表达有显著差异（P＜0．05）．PCNA在正常宫颈组织中无阳性表达，在宫颈鳞癌中的表达与宫颈不典型增生及正常宫颈组织有显著差异（P＜0．05），且随肿瘤恶性程度的增高而升高．bcl-2阳性表达产物定位于宫颈鳞癌细胞质，bcl-2在宫颈鳞癌Ⅰ，Ⅱ，Ⅲ各级中的表达有显著差异（P＜0．05），在宫颈鳞癌中的表达与宫颈不典型增生及正常宫颈有显著差异（P＜0．05）．结论：PCNA，bcl-2在子宫颈鳞状细胞癌中的过度表达及其共同作用，可能在宫颈鳞状细胞癌发生、发展过程有重要意义．
Objective: To investigate the expression of proliferating cell nuclear antigen (PCNA) and apoptosis-suppressing gene bcl-2 in cervical squamous cell carcinoma and its relationship with cervical squamous cell carcinoma. Methods: SP immunohistochemistry was used to detect 43 cases of cervical squamous cell carcinoma (Ⅰ grade in 10 cases, Ⅱ grade in 22 cases, Ⅲ grade in 11 cases), 24 cases of cervical Dysplasia, 16 cases of normal cervical tissue PCNA, bcl-2 gene product expression. Results: The expression of PCNA was significantly higher in cervical squamous cell carcinoma than that in cervical squamous cell carcinoma. The expression of PCNA in cervical squamous cell carcinoma Ⅰ, Ⅱ and Ⅲ was significantly different (P <0.05). There was no significant difference in PCNA expression between normal cervical tissues and cervical squamous cell carcinoma (P <0.05), but not with cervical dysplasia and normal cervix. The expression of PCNA increased with the malignant degree of cervical cancer. The expression of bcl-2 was localized in the cytoplasm of cervical squamous cell carcinoma. The expression of bcl-2 in cervical squamous cell carcinoma was significantly different (P <0.05), and the expression of bcl- Atypical hyperplasia and normal cervix have significant differences (P <0.05). Conclusion: The overexpression of PCNA and bcl-2 in cervical squamous cell carcinoma and their synergistic effect may play an important role in the occurrence and development of cervical squamous cell carcinoma.