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作者发现 Raji 细胞再感染 P3HR-1-EB 病毒产生的蛋白与 Burkitt 淋巴瘤病人血清作用能形成几个特异的免疫沉淀物。其他作者用免疫荧光法发现何杰金病及其他淋巴瘤、慢淋和急粒病人血清中有较高频率和滴度的 EB 病毒抗体存在。这些发现已扩大到毛细胞白血病。在此基础上,为了探讨毛细胞白血病病人血清中 EB 病毒抗体的存在及其特异性,作者用结合了放射自显影的 Laemmli SDS-聚丙酰胺梯度凝胶电泳系统作免疫沉淀,用~(14)C 乙酰化法测定分子量。以Raji 细胞再感染 P3HR-1-EB 病毒后细胞的各种膜碎
The authors found that Raji cells re-infected with P3HR-1-EB virus-producing protein and Burkitt lymphoma serum can form several specific immunoprecipitates. Other authors used immunofluorescence to detect Hodgkin’s disease and other Epstein-Barr virus, leiomyoma and acute myeloid serum in patients with higher frequency and titer of the presence of Epstein-Barr virus. These findings have expanded to hairy cell leukemia. On this basis, in order to investigate the presence and specificity of Epstein-Barr virus antibodies in sera from patients with hairy cell leukemia, the authors used immunoprecipitation with a Laemmli SDS-polyacrylamide gradient gel electrophoresis system incorporating autoradiography. C acetylation method for the determination of molecular weight. Various membrane fragments of cells after re-infection of P3HR-1-EB virus with Raji cells