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目的 :以人胎儿睾丸组织为供体 ,免疫缺陷小鼠为受体 ,研究人类睾丸生精细胞异种移植后的继续发育情况。 方法 :将 2 6周胎儿的睾丸组织植入去势裸鼠背部 ,于移植后 1 35d取出移植物 ,进行组织形态学观察 ,分析原始生精细胞在异种异位的发育情况。 结果 :移植 1 35d后取出的移植物显示 ,其生长幅度已由移植前直径约 1mm和湿重约 5mg ,分别增加到移植后大于 3mm和 2 0mg。组织形态学观察发现 ,移植前的睾丸主要是由直径为(6 0± 1 5 ) μm的精曲小管索构成 ,其中包含的细胞主要是原始Sertoli细胞和少量原始生精细胞 ,细胞排列呈弥散无规则状态 ;而移植后 1 35d的精曲小管索已发育成具有管腔的精曲小管 ,出现由Sertoli细胞和生精细胞组成的完整生精上皮 ,直径增大到 (80± 2 5 ) μm。原本呈不规则分布的原始Sertoli细胞和生精细胞大部分已迁移到基膜处 ,其中有少数生精细胞发育成为精原细胞。 结论 :人胎儿睾丸组织移植到去势裸鼠背部后 ,可以继续存活并进一步生长发育。
OBJECTIVE: To study the continuous development of human testicular spermatogenic cells after xenotransplantation using human fetal testes as donor and immunodeficient mice as recipients. Methods: Twenty-six weeks fetal testes were implanted into the back of castrated nude mice and the grafts were removed at 35 days after transplantation. Morphological changes were observed and the development of primary spermatogenic cells in xeno-heteroplasia was analyzed. Results: The grafts removed after 35 days of transplantation showed that the growth amplitude had increased from about 1 mm and wet weight about 5 mg before transplantation to more than 3 mm and 20 mg respectively after transplantation. Morphological observation showed that the testes before transplantation were mainly composed of fine curved tubules of (60 ± 1 5) μm in diameter. The cells contained were mainly primitive Sertoli cells and a few original spermatogenic cells, and the cells arranged in a diffuse manner And the intact tubule was composed of Sertoli cells and spermatogenic cells. The diameter of the seminiferous epithelium increased to (80 ± 2.5) μm. Originally irregular distribution of the original Sertoli cells and most of the spermatogenic cells have migrated to the basement membrane, in which a small number of germinal cells develop into spermatogonia. Conclusion: Human fetal testes can survive and grow further after transplanted to the back of castrated nude mice.