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目的：研究端粒酶活性与结直肠癌恶性进展的关系。方法：应用端粒重复扩增（ＴＲＡＰ）方法检测２２例大肠腺瘤、４３例结直肠癌及邻近组织中的端粒酶活性，并分析其与临床病理的关系。结果：端粒酶活性阳性率在大肠癌中为９５．４％，腺瘤中为９．１％，而邻近组织中仅为２．３％，在低、未分化癌及粘液腺癌中端粒酶活性呈中、强表达者占多，而在中、高分化癌包括乳头状腺癌及管状腺癌中存在相当低、弱表达，但其总的表达在大肠癌Ｄｕｋｅｓ各期中无甚差异。结论：大肠癌的恶性转化与端粒酶的活化有关，检测端粒酶活性对临床大肠癌尤其是早期大肠癌具有特别重要的诊断意义。
Objective: To study the relationship between telomerase activity and malignant progression of colorectal cancer. METHODS: Telomeric repeat amplification (TRAP) method was used to detect telomerase activity in 22 cases of colorectal adenoma, 43 cases of colorectal cancer and adjacent tissues, and analyze the relationship between the telomerase activity and clinical pathology. Results: The positive rate of telomerase activity was 95.4% in colorectal cancer, 9.1% in adenomas, and only 2.3% in adjacent tissues, in the low, undifferentiated, and mucinous adenocarcinomas. The expression of granzyme activity was moderately and strongly expressed, while it was low and weak in moderately and well-differentiated carcinomas, including papillary adenocarcinoma and tubular adenocarcinoma, but the total expression was not even in the Dukes stages of colorectal cancer. difference. Conclusion: The malignant transformation of colorectal cancer is related to the activation of telomerase. Detection of telomerase activity is of particular importance in the diagnosis of colorectal cancer, especially early colorectal cancer.