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目的:研究血管内皮生长因子(VEGF)以及地塞米松单独或联合应用对早产大鼠肺泡表面活性物质结合蛋白B(SP-B)mRNA表达的影响,及与肺发育的关系,为临床防治早产儿呼吸窘迫综合征(NRDS)提供新途径。方法:孕15d清洁级Wistar大鼠12只,随机分为4组,每组平均3只,试验分组:VEGF组、地塞米松组、VEGF加地塞米松组和对照组进行试验,应用RT-PCR检测早产鼠肺组织中SP-BmRNA的表达。结果:地塞米松组、VEGF加地塞米松组肺组织中SP-BmRNA表达与对照组比较,P<0.05,差异有显著性,VEGF加地塞米松组较地塞米松组SP-BmRNA表达进一步增加(P<0.05),而VEGF单独应用组与对照组比较,P>0.05,差异无显著性。结论:地塞米松能促进肺表面活性物质的合成和分泌,VEGF能协同地塞米松促进肺表面活性蛋白的分泌与合成。
Objective: To investigate the effects of vascular endothelial growth factor (VEGF) and dexamethasone alone or in combination on the expression of surfactant protein-B (SP-B) mRNA in lung of premature rats and their relationship with lung development, Children with respiratory distress syndrome (NRDS) provide a new way. Methods: Twelve pregnant Wistar rats were randomly divided into 4 groups (n = 3 each). The experimental groups were divided into three groups: VEGF group, dexamethasone group, VEGF plus dexamethasone group and control group. RT- The expression of SP-B mRNA in lung tissue of premature rats was detected. Results: Compared with the control group, the expression of SP-B mRNA in the dexamethasone group and the VEGF plus dexamethasone group was significantly lower than that in the control group (P <0.05). The expression of SP-B mRNA in the dexamethasone group and the dexamethasone group was more than that in the dexamethasone group P <0.05), while VEGF alone group compared with the control group, P> 0.05, no significant difference. Conclusion: Dexamethasone can promote the synthesis and secretion of pulmonary surfactant. VEGF can cooperate with dexamethasone to promote the secretion and synthesis of pulmonary surfactant protein.