论文部分内容阅读
为研究Ｐ物质激活的下丘脑大细胞神经分泌神经元及其分布，将Ｐ物质注入大鼠侧脑室，用Ｆｏｓ癌蛋白免疫组织化学结合催产素或加压素抗血清免疫组织化学的双标记法，检测了下丘脑各大细胞神经分泌核区被激活的催产素及加压素免疫反应性神经元。结果表明：Ｆｏｓ免疫强反应性的神经元主要见于第三脑室周围区及室旁核，在视上核及其他下丘脑大细胞神经分泌核区仅见到少数至极少数Ｆｏｓ免疫反应性神经元。Ｆｏｓ和催产素或加压素的免疫双染色神经元主要见于第三脑周围区，由于第三脑室周的加压素样神经元较少，因此在该区域我们见到的主要为中等至较多数量的Ｆｏｓ和催产素免疫双染色神经元，仅有少数的Ｆｏｓ和加压素免疫双染色神经元。室旁核的Ｆｏｓ免疫反应性神经元主要分布于室旁核的小细胞部，虽与催产素及加压素免疫反应性神经元的分布区有部分重叠，但该核区却仅见到少数Ｆｏｓ和催产素或加压素的免疫双染色神经元。此外，在视上核、背内侧副核也可见到少数Ｆｏｓ和催产素或加压素的免疫双染色神经元。本研究中将Ｐ物质注射入侧脑室后诱导的Ｆｏｓ阳性表达的催产素及加压素样神经元在下丘脑的分布与该区域Ｐ物质样传入纤维的分布不完全吻合，但与该区域Ｐ物质样受体的分布基本吻合。以上研究结果?
In order to study the substance P-stimulated hypothalamic neurons secreting neurons and its distribution, substance P was injected into the lateral ventricle of rats, and double-labeled immunohistochemistry with Fos oncoprotein combined with oxytocin or vasopressin antiserum , The oxytocin and vasopressin immunoreactive neurons activated by the secretory nucleus of the major neurons of the hypothalamus were examined. The results showed that Fos immunoreactive neurons mainly found in the periventricular and paraventricular nucleus of the third ventricle, only a few to very few Fos immunoreactive neurons were seen in the nucleus of supraoptic nucleus and other neurons of hypothalamic large cells. The immunostaining neurons of Fos and oxytocin or vasopressin are mainly found in the third perinuclear area. Since the number of vasopressin-like neurons in the third ventricle is small, we see mainly in this area are moderate to relatively The majority of Fos and oxytocin immunostain double stained neurons, only a few Fos and vasopressin-immunostained neurons. Paraventricular nucleus Fos immunoreactive neurons are mainly distributed in the paraventricular small cell, although with the distribution of oxytocin and vasopressin immunoreactive neurons are partially overlap, but only a handful of nuclear Fos And immunostaining neurons of oxytocin or vasopressin. In addition, in the supraoptic nucleus, a small number of immunostaining neurons of both Fos and oxytocin or vasopressin can also be seen in the dorsal medial subnucleus. In our study, the distribution of substance-loaded neurons in the hypothalamus induced by injecting substance P into the lateral ventricle was not completely consistent with the distribution of substance P-like fibers in this region, but not with the distribution of P Substance-like receptors are basically the same distribution. The above findings?