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目的探讨广东省汉族人群中抵抗素基因(RETN)的单核苷酸多态性(SNP)与2型糖尿病的关联。方法采用以医院为基础病例对照研究方法对2010年11月—2012年7月在广东省8家医院内分泌科就诊的595例2型糖尿病患者和同期在此8家医院健康体检的725名体检人群进行问卷调查,并采用SNPscanTM多重SNP分型试剂盒对筛选的2个tag SNP(rs7408174、rs3219175)进行基因分型;应用χ2检验、logistic回归模型等方法分析SNP位点与2型糖尿病的关联性。结果 rs7408174的TT、CT、CC基因型频数在病例组为329例(55.3%)、230例(38.7%)、36例(6.1%),在对照组为366人(50.8%)、308人(42.8%)、46人(6.4%);rs3219175位点的GG、GA、AA基因型频数在病例组为377例(63.4%)、200例(33.6%)、18例(3.0%),在对照组中为442人(61.0%)、253人(34.9%)、30人(4.1%);调整性别、体质指数和甘油三酯等因素后,rs7408174、rs3219175在等位基因关联分析和基因型关联分析中差异均无统计学意义(P>0.05)。结论广东省汉族人群rs7408174和rs3219175与2型糖尿病无关联。
Objective To investigate the relationship between single nucleotide polymorphism (SNP) of resistin gene (RETN) and type 2 diabetes in Han population of Guangdong province. Methods A case-control study based on a hospital-based approach was performed in 595 patients with type 2 diabetes mellitus who were treated in the endocrinology department of 8 hospitals in Guangdong province from November 2010 to July 2012 and 725 health examination people in the same period Two SNPs (rs7408174, rs3219175) were screened by using SNPscanTM multiple SNP typing kit. The relationship between SNPs and type 2 diabetes was analyzed by χ2 test and logistic regression model . Results The frequencies of TT, CT and CC genotypes in rs7408174 cases were 329 cases (55.3%), 230 cases (38.7%) and 36 cases (6.1%) in the case group and 366 (50.8%) and 308 42.8%) and 46 (6.4%). The frequency of genotypes GG, GA and AA in rs3219175 was 377 cases (63.4%), 200 cases (33.6%) and 18 cases (3.0% There were 442 (61.0%), 253 (34.9%) and 30 (4.1%) patients in the study group. After adjustment for gender, body mass index and triglyceride, rs7408174 and rs3219175 were significantly associated with allele association and genotype There was no significant difference in the analysis (P> 0.05). Conclusion There is no association between rs7408174 and rs3219175 in type 2 diabetes in Guangdong Han population.