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在原代培养的大鼠肾上腺嗜铬细胞上，综合运用细胞内钙测定法和全细胞膜片钳法，以检测膜电容变化为手段测定单一肾上腺嗜铬细胞的胞吐过程。－７０ｍＶ到＋２０ｍＶ去极化引起的钙电流和细胞膜电容的变化以及吹加６０ｍｍｏｌ／ＬＫＣｌ时，细胞内游离钙离子浓度［Ｃａ２＋］ｉ和细胞膜电容变化的同时检测，表明了Ｃａ２＋对细胞胞吐的控制作用。而用微碳纤电极则能检测到吹加６０ｍｍｏｌ／ＬＫＣｌ导致嗜铬细胞胞吐时儿茶酚胺的量子化释放。细胞膜电容检测和微碳纤电极检测从不同侧面动态的反映了细胞胞吐过程与Ｃａ２＋的相关性
In primary cultured adrenal chromaffin cells, intracellular calcium assay and whole-cell patch-clamp method were used to measure the change of membrane capacitance as a measure of exocytosis of single adrenal chromaffin cells. The changes of calcium current and membrane capacitance induced by depolarization at -70mV to + 20mV and the change of intracellular free calcium concentration [Ca2 +] i and the change of cell membrane capacitance when 60mmol / L KCl was blown in, indicated that Ca2 + Control effect. The use of micro-carbon fiber electrode can detect blowing plus 60mmol / LKCl lead to pheochromocytoma when excretion of catecholamine quantum release. Cell membrane capacitance detection and micro-carbon electrode detection from different aspects of dynamic reflect the process of cell exocytosis and Ca2 + correlation