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目的研究ＷＢＶ前Ｃ／Ｃ区基因变异的生物学意义。方法构建ＨＢＶ前Ｃ／Ｃ区基因ＥＢ病毒表达载体，采用脂质体介导方法将重组质粒转染到ＨｅｐＧ２细胞，并表达ＨＢｅＡｇ。结果重组质粒经ＰＣＲ和酶切鉴定均阳性，转染的细胞目的ＤＮＡ和表达蛋白检测均阳性。结论采用ＥＢ病毒载体构建ＨＢＶ前Ｃ／Ｃ基因的表达载体，能在ＨｅｐＧＺ细胞中稳定表达目的蛋白。由于ＥＢ病毒载体以附着体的形式复制，不整合于宿主染色体基因中，拷贝数稳定，适合于体外研究ＨＢＶＣ／Ｃ基因变异的生物学意义。
Objective To study the biological significance of pre-WB / C gene mutation in WBV. Methods The Epstein-Barr virus (EBV) expression vector of pre-HBV C / C gene was constructed. The recombinant plasmid was transfected into HepG2 cells by liposome-mediated method and HBeAg was expressed. Results The recombinant plasmids were positive by PCR and restriction enzyme digestion. The transfected cells were positive for DNA and protein expression. Conclusion The EBV vector was used to construct the pre-HBV C / C gene expression vector, which could stably express the target protein in HepG2 cells. Because of the replication of Epstein-Barr virus in the form of attachment, it is not integrated in the host chromosomal gene and the copy number is stable, so it is suitable for studying the biological significance of HBVC / C gene mutation in vitro.