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应用反转录－聚合酶链式反应（ＲＴ－ＰＣＲ）及限制性片段长度多态性（ＲＦＬＰ）和单链构象多态性（ＳＳＣＰ）分析技术，快速检测我国水稻条纹病毒（Ｒｉｃｅ ｓｔｒｉｐｅ ｖｉｒｕｓ， ＲＳＶ） ＲＮＡ４基因间隔区（ｉｎｔｅｒｓｅｎｉｃ ｒｅｇｉｏｎ，ＩＲ）的变异。 ＩＲ经 ＲＴ－ＰＣＲ扩增后得到一段长约 ６８０ ｂｐ的片段， ＲＳＶ ７个分离物之间片段大小相同，应用ＡｌｕＩ、 ＮｄｅＩ在它们之间检测到 ＲＦＬＰ，其中 ＢＳ、 ＪＤ、 ＬＹ、 ＹＬ分离物完全一致，而 ＳＱ、 ＰＪ、 ＪＮ分离物各不相同。 ＲＳＶ ７个分离物的 ＳＳＣＰ泳动带型证实了 ＲＦＬＰ分析结果。这是我国ＲＳＶ分子变异的首次报道。
The rapid detection of rice stripe virus (Rice stripe virus) in China by reverse transcription-polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP) and single strand conformation polymorphism (SSCP) RSV) RNA4 gene interspace (intersenic region, IR) variation. IR amplified a fragment of about 680 bp by RT-PCR. The fragment sizes of RSV 7 isolates were the same. RFLP was detected among them using AluI and NdeI, among which BS, JD, LY and YL isolates Exactly the same, while SQ, PJ, JN isolates vary. The SSCP bands of the RSV 7 isolates validated the RFLP analysis. This is the first report on the molecular variation of RSV in China.