论文部分内容阅读
目的:探讨浆细胞样树突状细胞(pDC)、干扰素α(IFN-α)及CD4+CD25+调节性T细胞(Treg细胞)在Graves病(GD)中的免疫致病机制。方法:收集GD患者及正常对照者的外周血及甲状腺组织,利用实时PCR、免疫组化、流式细胞仪、细胞分选及纯化等技术对组织或体外实验中的细胞数目、mRNA水平或蛋白水平进行分析比较。结果:GD患者中外周血血清IFN-α水平升高,分泌IFN-α的pDC亚群细胞数目增加;IFN-α还可以诱导甲状腺细胞表达IFN-α诱导基因(IFIGs)、人类白细胞抗原(HLA)-DR基因和促甲状腺激素受体(TSHR)基因的mRNA;GD患者外周血中Treg细胞比例降低;DC使Treg细胞更易凋亡。结论:GD的发病与pDC比例增多、IFN-α水平升高以及Treg细胞比例下降等多种免疫调节因素相关。
Objective: To investigate the immunopathogenesis of plasmacytoid dendritic cells (pDC), interferon alpha (IFN-α) and CD4 + CD25 + regulatory T cells (Tregs) in Graves disease (GD). Methods: Peripheral blood and thyroid tissues of GD patients and normal controls were collected. The number of cells, mRNA level or protein in tissue or in vitro experiments were detected by real-time PCR, immunohistochemistry, flow cytometry, cell sorting and purification Level analysis and comparison. Results: The levels of IFN-α in peripheral blood of GD patients increased and the number of pDC subsets secreting IFN-α increased. IFN-α also induced the expression of IFN-α induced genes (IFIGs), human leukocyte antigen ) -DR gene and thyrotropin receptor (TSHR) gene mRNA; GD patients with decreased proportion of Treg cells in the peripheral blood; DC Treg cells more susceptible to apoptosis. Conclusion: The incidence of GD is associated with many immunoregulatory factors such as increased proportion of pDC, elevated IFN-α and decreased proportion of Treg cells.