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DNA是具有遗传特性的物质基础,其损伤后的修复合成功能与细胞的癌变、遗传性疾病、衰老都有密切关系。现已发展成为遗传医学的新领域,分子遗传学研究的重要内容。1971年Lieberman应用静止的无分裂能力的淋巴细胞研究DNA修复合成,有利于区别修复合成和半保留复制合成。随后Pero和Capelli等继续研究和应用于化学物致突变性检测。但目前此方法在推广应用上尚有困难。为此我们对Lieberman法进行了简化,应用简化的方法研究了化学致突物诱发淋巴细胞DNA修复合成,并首次测定了正常人群淋巴细胞DNA修复合成,Unscheduled DNA Synthesis,简称UDS。
DNA is a material basis with genetic characteristics, and its repair function after injury is closely related to the carcinogenesis, hereditary diseases and aging of cells. Has now developed into a new field of genetic medicine, molecular genetics an important part of the study. In 1971, Lieberman applied quiescent, non-dividing lymphocytes to study DNA repair synthesis that favored the differential repair of synthetic and semi-preserved replication. Subsequently, Pero and Capelli et al continue to study and apply to the detection of chemical mutagenicity. However, this method is still difficult to popularize and apply. For this reason, we simplified Lieberman’s method and studied the synthesis and DNA synthesis of lymphocyte DNA induced by chemoremins by using the simplified method. We also measured the DNA repair synthesis, Unscheduled DNA Synthesis (UDS) of lymphocytes in normal population for the first time.