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目的:探讨CCCTC-结合因子(CCCTC-binding factor,CTCF)在子宫内膜癌中的表达及临床诊断意义。方法:随机选取30例正常子宫内膜标本(其中15例增殖期内膜组织,15例分泌期内膜组织)为对照组,45例子宫内膜癌患者的子宫内膜标本为实验组。采用Western blot法检测CTCF蛋白和人端粒酶逆转录酶(human telomerase reverse transcriptase,hTERT)在各组中的表达水平以及相关性;半定量RT-PCR法检测各组中CTCF基因mRNA及hTERT基因mRNA转录水平。结果:CTCF和hTERT在各组中均有表达。实验组CTCF蛋白和hTERT基因蛋白阳性表达率及相对表达量与对照组(分别88.89%vs.60%χ2=8.57,P=0.005;80%vs.26.67%,χ2=21.11,P=0.000;t=50.039,P=0.000;t=10.662,P=0.000)间存在差异;实验组CTCF基因mRNA和hTERT基因mRNA相对表达量与对照组(分别t=67.448,P=0.000;t=65.908,P=0.000)间同样具有统计学差异性。CTCF的表达与子宫内膜癌的病理分级和临床手术分期均有关(分别χ2=8.613,P=0.007;χ2=11.739,P=0.001),与病理类型无关(χ2=4.906,P=0.086);hTERT的表达与子宫内膜癌病理分级、病理类型、临床分期均无关(分别χ2=0.352,P=0.258;χ2=1.568,P=0.457;χ2=0.000,P=1.000)。子宫内膜癌中CTCF和hTERT的异常表达存在相关性(χ2=5.625,P=0.018),并呈正相关(r=0.354,P=0.017)。结论:CTCF与端粒酶hTERT在正常子宫内膜组织与子宫内膜癌中表达具有差异性,不同临床参数下CTCF与hTERT表达情况各异,CTCF和hTERT在预测与临床参数关系密切的子宫内膜癌复发过程中起重要作用。
Objective: To investigate the expression of CCCTC-binding factor (CTCF) in endometrial carcinoma and its clinical significance. Methods: Thirty normal endometrium specimens (including 15 proliferative endometrial tissues and 15 secretory endometrial tissues) were randomly selected as the control group. The endometrial specimens from 45 patients with endometrial carcinoma were selected as the experimental group. The expression of CTCF protein and human telomerase reverse transcriptase (hTERT) in each group were detected by Western blot and the correlation was analyzed by semi-quantitative RT-PCR. The expression of CTCF mRNA and hTERT gene mRNA transcription level. Results: CTCF and hTERT were expressed in all groups. The positive rate and relative expression of CTCF protein and hTERT gene protein in the experimental group were significantly higher than those in the control group (88.89% vs.60% χ2 = 8.57, P = 0.005; 80% vs.26.67%, χ2 = 21.11, P = 0.000; = 50.039, P = 0.000; t = 10.662, P = 0.000). The relative expression levels of CTCF mRNA and hTERT mRNA in the experimental group were significantly higher than those in the control group (t = 67.448, P = 0.000; 0.000) also have statistical differences. The expression of CTCF was related to the pathological grade and clinical stage of endometrial cancer (χ2 = 8.613, P = 0.007; χ2 = 11.739, P = 0.001, respectively) The expression of hTERT was not associated with the pathological grade, pathological type and clinical stage of endometrial carcinoma (χ2 = 0.352, P = 0.258; χ2 = 1.568, P = 0.457, χ2 = 0.000, P = 1.000). There was a positive correlation between abnormal expression of CTCF and hTERT in endometrial carcinoma (χ2 = 5.625, P = 0.018) (r = 0.354, P = 0.017). Conclusion: The expression of CTCF and telomerase hTERT in normal endometrium and endometrial carcinoma are different. The expressions of CTCF and hTERT are different in different clinical parameters. CTCF and hTERT are different in the intrauterine uterus predicted to be closely related to clinical parameters Membrane cancer recurrence plays an important role in the process.