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目的 :探讨营养不良、肾病本身和糖皮质激素作为各自独立因素对大鼠血清胰岛素样生长因子结合蛋白 (IGFBPs)浓度的影响 ,阐明血清IGFBPs代谢紊乱与肾病综合征 (NS)大鼠生长障碍的关系。 方法 :2 4只周龄相同体重相近的雄性SD大鼠被随机分成正常、食物对照、阿霉素 (5mg/kg)肾病和地塞米松 (1 8mg/kg·d-1)治疗的阿霉素肾病四组。血清IGFBPs浓度和肝脏IGFBPsmRNA表达分别采用Westernligandblot和RT PCR法检测。 结果 :①食物对照和肾病组大鼠血清IGFBP 3浓度均减低 ,且肾病组更明显 ;激素治疗组较肾病组则增高。食物对照组大鼠血清IGFBP 2浓度也减低 ,肾病和激素治疗组却显著增高 ,但后两组间差异不显著。②食物对照组大鼠肝脏IGFBP 2mRNA表达下降 ,肾病组升高 ,激素治疗组较肾病组减低。食物对照组大鼠肝脏IGFBP 3mRNA表达正常 ,肾病组减低 ,激素治疗组较肾病组进一步下降。③血清IGFBP 2浓度与大鼠鼻 尾长度和体重均呈负相关 (P均 <0 0 1)。 结论 :肾病本身所致的血清IGFBP 2浓度增高是NS大鼠生长障碍发生的重要因素之一。
Objective: To investigate the effect of malnutrition, nephrosis and glucocorticoids on serum insulin - like growth factor binding protein (IGFBPs) concentrations in rats and to elucidate the relationship between serum IGFBPs metabolic disorders and growth disorders in rats with nephrotic syndrome relationship. METHODS: Twenty-four male Sprague-Dawley rats of similar body weight at the same age were randomly divided into normal, control, doxorubicin (5mg / kg) nephropathy and dexamethasone (18mg / kg · d-1) Four groups of kidney disease. Serum IGFBPs concentration and liver IGFBPsmRNA expression were detected by Westernligandblot and RT PCR. Results: ① The levels of serum IGFBP3 in food control group and nephrosis group were decreased, and the nephrosis group was more obvious. The hormone therapy group was higher than the nephropathy group. Serum IGFBP 2 concentrations also decreased in the food control group, but were significantly increased in the nephropathy and hormone treatment groups, but not significantly different between the two groups. ② The food control group rats liver IGFBP 2mRNA expression decreased, nephropathy group increased hormone treatment group than the nephropathy group decreased. The food control group rats liver IGFBP3mRNA expression was normal, nephropathy group decreased hormone treatment group compared with the nephropathy group decreased further. ③ Serum IGFBP 2 concentration was negatively correlated with the length and body weight of rat’s nose and tail (P <0.01). Conclusion: Increased serum IGFBP 2 levels caused by nephropathy are one of the important factors in the growth retardation of NS rats.