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目的建立巴豆非脂肪油部分的高效液相色谱指纹图谱,为其质量评价提供理论依据。方法采用RP-HPLC法,测定了10批巴豆样品。色谱条件:Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),流动相:A相为0.2%醋酸,0.3%三乙胺溶液,B相为乙腈,梯度洗脱,体积流量:0.8 mL/min,柱温:30℃,检测波长:247 nm。结果在色谱指纹图谱中,确立了其中12个共有峰,建立了巴豆非脂肪油部分的共有模式,10批样品指纹图谱相似度在0.982～0.995之间。结论该方法简便、准确、重现性好,为评价巴豆药材的质量提供了依据。
OBJECTIVE To establish HPLC fingerprint of non-fatty oil in Croton to provide a theoretical basis for its quality evaluation. Methods Ten batches of crudis samples were determined by RP-HPLC. The chromatographic conditions were as follows: Agilent Eclipse XDB-C18 column (250 mm × 4.6 mm, 5 μm), the mobile phase consisted of 0.2% acetic acid in phase A, acetonitrile in 0.3% acetonitrile and gradient elution in phase B mL / min, column temperature: 30 ℃, detection wavelength: 247 nm. Results In chromatographic fingerprinting, 12 common peaks were established, and the common mode of non-fatty part of croton was established. The fingerprint similarity of 10 batches of samples was between 0.982 and 0.995. Conclusion The method is simple, accurate and reproducible, which provides the basis for the quality evaluation of croton medicine.