产单核细胞李斯特菌是一种兼性细胞内快速生长的细菌,能引起食物中毒并在免疫受损的个体中导致严重的疾病。其主要的毒力决定簇是产生本质为穿孔蛋白的李斯特菌溶解素O(LLO),LLO是细菌从宿主空泡中逸出所必需的物质。然而,人们对LLO在细菌于胞内生长以及胞间传播过程中的作用尚未进行测定。作者将纯化的LLO与LLO阴性的李斯特菌突变株结合,可观察到细菌从空泡中逸出。 Listeria monocytogenes is a rapidly growing bacterium in facultative cells that causes food poisoning and causes serious illness in immunocompromised individuals. Its major virulence determinant is the production of Listeria Soluble O (LLO), an essential perforin protein that is essential for bacteria to escape from host vacuoles. However, the role of LLOs in the intracellular growth and intercellular transmission of bacteria has not yet been determined. The authors combined purified LLO with LLO-negative Listeria mutans, and observed the escape of bacteria from the vacuole.