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目的 观察荧光定量聚合酶链反应 (PCR)PE5 70 0测定丙型肝炎病毒RNA的准确性。方法 所用临床评价血清盘由 36份血清组成 ,其中包括 1个 5倍倍比稀释系列 (7份 )、9份阴性血清和 2 0份阳性血清。使用临床评价血清盘比较 2种测定方法的重复性、线性和临床样本符合情况。结果 实时荧光定量方法和全自动PCR ELISA内标法检测系统的线性回归系数分别为 0 .9732和0 .9995。全自动PCR酶联免疫吸附 (ELISA)内标法检测系统不同含量的样本批内变异系数 (log10 )均比实时荧光定量方法低。临床样本的检测实时荧光定量方法与全自动PCR ELISA内标法检测系统的相关系数为 0 .845。结论 实时荧光定量方法具有良好的线性和重复性 ,同时与全自动PCR ELISA内标法检测系统有良好的相关性
Objective To observe the accuracy of fluorescent quantitative polymerase chain reaction (PCR) PE5 70 0 for the determination of hepatitis C virus RNA. Methods The clinical evaluation of the serum disk used consisted of 36 sera, including one 5-fold dilution series (7), 9 negative sera and 20 positive sera. The reproducibility, linearity, and clinical sample comparisons of the two assays using the clinical evaluation of the serum disk fit the situation. Results The linear regression coefficients of the real-time fluorescence quantitative assay and the automated PCR-ELISA internal standard assay system were 0.9732 and 0.9995, respectively. The intra-assay coefficient of variation (log10) of the system with different contents of internal standard ELISA was lower than that of the real-time fluorescence quantitative assay. The correlation coefficient between real-time fluorescence quantitative method of clinical samples and automatic PCR ELISA internal standard method was 0.885. Conclusion The real-time fluorescence quantitative method has good linearity and repeatability, and has good correlation with the automatic PCR ELISA internal standard detection system