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用晶体培养方法研究硒与光照射诱发的晶体脂质过氧化作用的关系。结果表明经光照射24~45h后晶体丙二醛(MDA)含量显著升高(P<0.05)。培养液中含适宜硒浓度(1.15μmol/L)可使光照射晶体的丙二醛含量减少,并在一定硒浓度范围内经光照射晶体的丙二醛含量与培养液中硒浓度呈负的剂量反应关系。两组在不同硒浓度培养液中培养的晶体经光照射后谷胱甘肽过氧化物酶(GSH-Px),超氧化物歧化酶(SOD)的活性无明显差别。推测硒能抑制光照射诱发的晶体脂质过氧化作用,硒对晶体的保护可能是通过谷胱甘肽过氧化物酶以外的其它途径。
Study on the Relationship between Selenium and Lipid Peroxidation Induced by Light Irradiation by Crystal Culture. The results showed that the content of malondialdehyde (MDA) was significantly increased after 24 ~ 45h light exposure (P <0.05). The concentration of malondialdehyde (1.15μmol / L) in the culture solution could decrease the content of malondialdehyde in the light-irradiated crystal and the content of malondialdehyde in the light-irradiated crystal within a certain range of selenium concentration was negative with the concentration of selenium in the culture solution Dose response relationship. There was no significant difference in the activity of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) between the two groups cultured in different concentrations of selenium. Speculated that selenium can inhibit the light-induced lipid peroxidation of the crystal, selenium on the crystal may be protected by other means than glutathione peroxidase.