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为了解安徽省幼儿园儿童隐孢子虫感染情况及流行特点,进行了此项调查,结果简报如下。 1.对象与方法:按随机化原则,以班为单位进行整群抽样,于2001年5～10月在安徽省内11个地、市29所幼儿园共对1204名儿童(年龄3～6岁,平均年龄4.39岁,其中男性儿童641人,女性儿童563人;城市儿童684人,乡镇儿童520人)粪便标本进行检测。粪便标本采用金胺-酚染色法、改良抗酸染色法、沙黄-美蓝染色法、金胺-酚改良抗酸复染法4种方法染色,显微镜下检查隐孢子虫卵囊。
In order to understand the prevalence and epidemiological characteristics of children’s Cryptosporidium in kindergartens in Anhui Province, this survey was conducted and the results are summarized as follows. 1. Subjects and Methods: According to the principle of randomization, cluster sampling was conducted in units of classes. From May to October in 2001, 11 kindergartens in 29 places in Anhui Province and 29 kindergartens in total participated in the investigation of 1204 children (aged 3 to 6 years , The average age of 4.39 years, of which 641 male children, 563 female children; 684 urban children, township children 520) stool specimens were detected. The stool samples were stained with four methods of Auramine - phenol staining, modified acid - fast staining, sand yellow - methylene blue staining and Auramine - phenol modified acid - fast staining. The Cryptosporidium oocysts were examined under the microscope.