This study has estimated the growth and mortality parameters of some important fishspecies including Croaker Atrobucca alcocki，Tiger toothed croaker Otolithes cuvieri，andRibbon fish Lepturacanthus savala using the length-frequency data in Pakistani waters.However the length-weight relationship (LWR)，biological reference points (BRPs) were alsoestimated.The length-frequency data were collected from the fishery resources survey ofPakistan during the year 2009-2010.The Maximum sustainable yield were also estimated ofBarramundi，Lates calcarifer and Black Pomfret Parastromateus niger using the catch andeftort data taken from the fisheries statistics book of Pakistan complied by Marine FisheriesDepartment (MFD) Government of Pakistan，Karachi.　　Atrobucca alcocki　　The fisheries statistics book of Pakistan is evident that prior to the fishery resourcessurvey of Pakistan during the year 2009-2010，the Atrobucca alcocki were not observed inthat abundance as observed in surveys during 2009-2010.The species was observed in auniform abundance during the four demersal trawl surveys and it was the main objective ofour study to estimate the growth and mortality parameters of this specie.In this connection atotal of 819 (n =819) length weight and 7240 (n=7240) length frequency of Atrobuccaalcocki were observed，the minimum length were 10cm and maximum length were 44cmwith the dominant length group ranges from 19 to 29cm.The total weight ranges from 14 to928g.The length-weight relationship parameters were estimated as a =0.012，b =2.925andR2 =0.972.Applying FiSAT computer software package using ELEFAN method the length-frequency distribution data were analyzed.The calculated von Bertalanffy growth functionparameters were L∞=47.25cm，K=0.180 yr-1.Total mortality (Z) were computed using thelength-converted catch curve analysis at Z=1.07yr-1.Natural mortality using Paulys equationwere computed as M=0.494 yr-1 at an annual average sea surface temperature of 26 0℃，hencethe fishing mortality were computed as F=Z-M=0.576 yr-1.Yield per recruit analysis revealedthat when tc was assumed to be 2，Fmax was calculated at 0.75 and F0.1 at 0.6.When tc wasassumed to be 1，Fmax was calculated at 0.55 and F0.1 at 0.45.Current age at first capture isabout 1 year and Fcurrent was 0.576 therefore Fcurrent was larger than F0.1 and Fmax whichshows that the fishery ofAtrobucca alcocki may not be in a sustainable state in the region.Hence it is recommended that fishing effort may be reduced for the rational exploitation ofthe species in the region.　　Otolithes cuvieri　　Although 66 genera and 284 species belonging to the family Sciaenidae aredistributed worldwide but in Pakistani waters 18 species belongs to family Sciaenidae arewell known，but the catch in such abundance of Otolithes cuvieri were observed first timeduring the fisheries resources survey of Pakistani waters during the year 2009-2010.Keepingthis in view we had put an attempt to estimate the growth and mortality parameters of theOtolithes cuvieri using the length-frequency data.Four demersal trawl surveys were carriedout in Pakistani marine waters for the collection of length-frequency data of the species.Thedata used in present study for the combined sexes of the species were based on 3219 length-frequency (n=3219) and 671 length-weight (n=671) pairs.The length ranges from 3-35cmand weight ranges from l-208g.The dominant length of Otolithes cuvieri ranges from 4-18cm.The length weight relationship parameters were computed as a=0.014，b=2.870 andR2 =0.952.Using the ELEFAN routine of the FiSAT computer package，the calculated vonBertalanffy growth function parameters were L∞ =36.75cm，K=0.300year-1.Total mortalityrate (Z) were computed using length-converted catch curve analysis at Z=1.26 year-1.Following the Paulys equation natural mortality rate were computed as M=0.740year-1，atan average sea surface temperature of 260 ℃，fishing mortality rate were computed as F=Z-M=0.52 year-1.The yield per recruit analysis showed that when tc was 1，Fmax wascalculated at 0.5 and F0.1 at 0.4.When biological reference point of Fopt was M (0.740),current fishing mortality rate of 0.52 is smaller than the target biological reference point.Thisindicates that the fishery is in a safe condition.　　Lepturacanthus savala　　Lepturacanthus savala (Cuvier，1829) is a commercial fish species distributed in thecoastal waters of Indo-west Pacific and Indian Ocean.In present study Length-frequency datawere analyzed.The length-frequency data of Lepturacanthus savala (Cuvier 1829) werecollected by the four demersal trawl surveys in the Pakistani waters in 2009-2010.A total of4035 pairs of lengths and 1391 pairs of length-weight were measured.The length ranges from1 to 121cm with average length of 44.23cm (± 19.75cm) while weight ranges from 1 to1942g with average weight of 110.81 g (±229.57g).The estimated values of length-weightrelationship for L.savala were W =0.0001*L？191 (R2 =0.960).The calculated yon Bertalanffygrowth function parameters using ELEFAN method in FiSAT computer package ofL.savalawere L∞ =133.35 cm and k =0.130year-1，t0 were estimated at-0.877.The estimated rate oftotal mortality Z applying the length-converted catch curve analysis method for L.savala wasZ =0.49year-1，natural mortality M were estimated as M=0.304year-1 at an annualaverage sea surface temperature of 26 ℃ while the rates of fishing mortality F was calculatedas F=0.185year-1.Hence exploitation ratio (E=F/Z) were calculated as 0.377 yr-1.Yield perrecruit contour map reveals that when tc was assumed to be 1，Fmax was estimated at 0.6 andF0.1 at 0.45.Current age at first capture was about 1 year and Fcurrent was 0.185 thereforeFcurent was smaller than Fo1 and Fmax.When biological reference point Fopt was equal to M(0.304)，current fishing mortality rate of 0.185 is smaller than the target biological referencepoint.The estimated values of growth performance index for the L.savala from Pakistaniwaters wereφ =3.364year-1.The estimated value of MSY was 26983t with the estimatedbiomass of 110135t.Again the obtained results of maximum sustainable yield (MSY) of26983t for L.savala from Pakistani waters were larger than recent catch of 20375t; thereforewe may indicate that the L.savala fishery in Pakistani may be in a sustainable state.　　Lates calcarifer (MSY)　　This chapter was to estimate the maximum sustainable yield of the Barramundi Latescalcarifer because it is a commercially important fish species and was fished in abundance in1980s from the southern part of the Indus delta but in recent years it is caught not in thatabundance as in 1980s.Maximum Sustainable Yield (MSY) of Barramundi Lares calcarifer(Bloch，1790) fishery of Pakistan were estimated based on catch and effort data from 1995-2009.Two computer software packages CEDA (catch effort data analysis) and ASPIC (asurplus production model incorporating covariates) were applied.Three surplus productionmodels including Fox，Schaefer and Pell-Tomlinson with three error assumptions of normal,log-normal and gamma were used by applying CEDA and two surplus production modelsincluding Fox and Schaefer were used by applying ASPIC computer software package.InCEDA initial proportion (IP) was used ranging from 0.1-09.The obtained MSY results using1P 0.8 (because the initial catch was roughly 80％ of the maximum catch) in normal，log-normal and gamma error assumptions from Fox，Schaefer and Pella-Tomlinson models wereabout 107-117t (± 8.131-15.260)t and estimated R2 for three models with three errorassumptions were about 0.61-0.67.The estimated MSY results of Fox and Schaefer modelsusing ASPIC computer software package were 118t (±10.856)t and 109t (± 10.384)t andestimated R2 for Fox and Schaefer models were 0.803 and 0.794 respectively.The estimatedvalues of MSY are smaller than the recent catch which indicates that the fishery of L.calcarifer in Pakistani waters is over-exploited.Hence it is recommended to the fisherymanagers that for the sustainable exploitation of the species in Pakistani waters fishing effortshould be reduced.　　Parastromateus niger (MSY)　　Parastromateus niger is among the most important commercial fish species in thePakistan.The main emphasis of this chapter was to evaluate the MSY maximum sustainableyield of Parastromateus niger fishery based on the catch and effort data from 1992-2009.The computer software package CEDA (catch effort data analysis) was used to analyze thecatch and effort data for the estimation of maximum sustainable yield (MSY) ofParastromateus niger fishery from Pakistani waters.An initial proportion IP of 0.4 (becausethe initial catch was roughly 40％ of the maximum catch) were applied，the estimatedmaximum sustainable yield (MSY) were about 2000-2300t，the correlation coefficient R2 wereabout 0.20-0.31.Gamma error assumption often showed minimization failure.The estimatedMSY from CEDA is smaller than most recent catch of the data which indicates that the fisheryof Black Pomfret P niger in Pakistani waters may not be in a sustainable condition.Hence itis suggested that，for the well being and sustainable utilization of the fishery，the fisherymanagement authorities and other stack holder may take some necessary actions to ensure thesustainable exploitation of the species in the region for coming generations.